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Optimization and single-laboratory validation study of a high-performance liquid chromatography (HPLC) method for the determination of phenolic Echinacea constituents

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Abstract

Three species of Echinacea (Echinacea purpurea, Echinacea angustifolia, and Echinacea pallida) are commonly used for medicinal purposes. The phenolic compounds caftaric acid, cichoric acid, echinacoside, cynarin, and chlorogenic acid are among the phytochemical constituents that may be responsible for the purported beneficial effects of the herb. Although methods for the analysis for these compounds have been published, documentation of their validity was inadequate as the accuracy and precision for the detection and quantification of these phenolics was not systematically determined and/or reported. To address this issue, the high-performance liquid chromatography method, originally developed by the Institute for Nutraceutical Advancement (INA), was reviewed, optimized, and validated for the detection and quantification of these phenolic compounds in Echinacea roots and aerial parts.

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Acknowledgments

We would like to thank our co-operative student Anna Chow for her assistance in carrying out this project. We would also like to acknowledge and thank Andy Renz and Ron Smith, Natural Factors Nutritional Products Ltd., for their assistance on this project.

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Authors and Affiliations

  1. British Columbia Institute of Technology, 3700 Willingdon Avenue, Burnaby, BC, V5G-3H2, Canada

    Paula N. Brown & Michael Chan

  2. Office of Dietary Supplements, National Institutes of Health, Bethesda, MD, 20892, USA

    Joseph M. Betz

Authors
  1. Paula N. Brown
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  2. Michael Chan
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  3. Joseph M. Betz
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Correspondence to Paula N. Brown.

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Brown, P.N., Chan, M. & Betz, J.M. Optimization and single-laboratory validation study of a high-performance liquid chromatography (HPLC) method for the determination of phenolic Echinacea constituents. Anal Bioanal Chem 397, 1883–1892 (2010). https://doi.org/10.1007/s00216-010-3763-z

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  • DOI: https://doi.org/10.1007/s00216-010-3763-z

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