Analytical and Bioanalytical Chemistry

, Volume 394, Issue 8, pp 2241–2247

Rapid analysis of organic farming insecticides in soil and produce using ultra-performance liquid chromatography/tandem mass spectrometry

Authors

    • Institute of Plant Protection, National Research Institute
  • Jolanta Kowalska
    • Institute of Plant Protection, National Research Institute
Original Paper

DOI: 10.1007/s00216-009-2931-5

Cite this article as:
Drożdżyński, D. & Kowalska, J. Anal Bioanal Chem (2009) 394: 2241. doi:10.1007/s00216-009-2931-5

Abstract

A new method for the analysis of three ecological insecticides, namely azadyrachtin, spinosad (sum of spinosyn A and spinosyn D) and rotenone, in produce and soil samples is presented. Investigated compounds are one of the most significant insecticides authorized for organic farming crop protection in many countries. Extraction of the pesticides from plant and soil matrices was performed by using a modified quick, easy, cheap, effective, rugged, and safe (QuEChERS) method. The method entailed a single extraction of the investigated compounds with acidified acetonitrile followed by a dispersive solid-phase extraction cleanup step prior to the final determination by reverse-phase ultra-performance liquid chromatography/tandem quadrupole mass spectrometry (UPLC-MS/MS). Validation studies were carried out on cabbage, tomato and soil samples. Recoveries of the spiked samples were in the range between 67% and 108%, depending on the matrix and the spiking level. Relative standard deviations for all matrix–compound combinations did not exceed 12%. The limits of quantification were ≤0.01 mg kg−1 in all cases, except for azadirachtin. The developed method was applied to the analysis of real samples originating from organic farming production.

Keywords

Ecological insecticides Soil Food QuEChERS UPLC-MS/MS

Copyright information

© Springer-Verlag 2009