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Metabolism and toxicological detection of the designer drug 4-chloro-2,5-dimethoxyamphetamine in rat urine using gas chromatography-mass spectrometry

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Abstract

Studies are described on the metabolism and the toxicological analysis of the amphetamine-derived designer drug 4-chloro-2,5-dimethoxyamphetamine (DOC) in rat urine using gas chromatographic-mass spectrometric techniques. The metabolites identified indicated that DOC was metabolized by O-demethylation at position 2 or 5 of the phenyl ring partly followed by glucuronidation and/or sulfation. The authors’ systematic toxicological analysis procedure using full-scan gas chromatography-mass spectrometry after acid hydrolysis, liquid-liquid extraction and microwave-assisted acetylation allowed the detection of an intake of a dose of DOC in rat urine that corresponds to a common drug user’s dose. Assuming similar metabolism, the STA procedure described should be suitable as proof of an intake of DOC in human urine.

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Acknowledgements

The authors would like to thank Johanna Schäning, Frank T. Peters, Christoph Sauer, Gabi Ulrich and Armin A. Weber for their suggestions and help.

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Authors and Affiliations

  1. Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, 66421, Homburg (Saar), Germany

    Andreas H. Ewald & Hans H. Maurer

  2. cc chemical consulting, Schoenburgstr. 2, 12103, Berlin, Germany

    Dorothea Ehlers

Authors
  1. Andreas H. Ewald
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  2. Dorothea Ehlers
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  3. Hans H. Maurer
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Correspondence to Hans H. Maurer.

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Ewald, A.H., Ehlers, D. & Maurer, H.H. Metabolism and toxicological detection of the designer drug 4-chloro-2,5-dimethoxyamphetamine in rat urine using gas chromatography-mass spectrometry. Anal Bioanal Chem 390, 1837–1842 (2008). https://doi.org/10.1007/s00216-008-1917-z

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  • DOI: https://doi.org/10.1007/s00216-008-1917-z

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