Analytical and Bioanalytical Chemistry

, Volume 389, Issue 1, pp 63-76

First online:

Analytical procedures for water-soluble vitamins in foods and dietary supplements: a review

  • Christopher J. BlakeAffiliated withFood Quality and Safety Department, Nestlé Research Centre Email author 

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Water-soluble vitamins include the B-group vitamins and vitamin C. In order to correctly monitor water-soluble vitamin content in fortified foods for compliance monitoring as well as to establish accurate data banks, an accurate and precise analytical method is a prerequisite. For many years microbiological assays have been used for analysis of B vitamins. However they are no longer considered to be the gold standard in vitamins analysis as many studies have shown up their deficiencies. This review describes the current status of analytical methods, including microbiological assays and spectrophotometric, biosensor and chromatographic techniques. In particular it describes the current status of the official methods and highlights some new developments in chromatographic procedures and detection methods. An overview is made of multivitamin extractions and analyses for foods and supplements.


Water-soluble vitamins Foods Dietary supplements Microbiological assay Chromatography Biosensor