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The use of gold bands for flow immunoelectrochemical devices

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Abstract

Gold bands sputtered over a polymeric material, Kapton, have been employed not only for electrochemical detection but also for the development of enzyme immunoassays in a flow system. The immunological interactions on bands acting as reactors are considered for a model analyte, IgM. Different formats of flow immunoassays, competitive and non-competitive, have been checked. Compared with previous results, automation gives rise to in a reduction in analysis time and in reagent consumption. Lower limits of detection are also obtained. Detection, which is also carried out in the flow system, is based on the oxidation of naphthol, the product of the enzymatic hydrolysis of naphthyl-phosphate.

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Acknowledgements

This work was supported by Project BIO2000–0588 (Plan Nacional de I+D+I). Eva M. Abad Villar thanks Universidad de Oviedo for the award of a Ph.D. grant. The authors also want to thank Professor José María Alameda Maestro and members of his group in the Departamento de Física de la Materia Condensada de la Universidad de Oviedo for help in the gold sputtering process.

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Authors and Affiliations

  1. Departamento de Química Física y Analítica, Universidad de Oviedo, Asturias, Spain

    Eva M. Abad-Villar, M. Teresa Fernández-Abedul & Agustín Costa-García

Authors
  1. Eva M. Abad-Villar
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  2. M. Teresa Fernández-Abedul
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  3. Agustín Costa-García
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Correspondence to Agustín Costa-García.

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Abad-Villar, E.M., Fernández-Abedul, M.T. & Costa-García, A. The use of gold bands for flow immunoelectrochemical devices. Anal Bioanal Chem 377, 267–272 (2003). https://doi.org/10.1007/s00216-003-2122-8

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  • DOI: https://doi.org/10.1007/s00216-003-2122-8

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