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Knockout of arsenic (+3 oxidation state) methyltransferase results in sex-dependent changes in phosphatidylcholine metabolism in mice

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Abstract

Arsenic (+3 oxidation state) methyltransferase is the key enzyme in the methylation pathway for inorganic arsenic. We have recently shown that As3mt knockout (KO) has a profound effect on metabolomic profiles in mice. Phosphatidylcholine species (PCs) were the largest group of metabolites altered in both plasma and urine. The present study used targeted analysis to investigate the KO-associated changes in PC profiles in the liver, the site of PC synthesis. Results show that As3mt KO has a systemic effect on PC metabolism and that this effect is sex dependent.

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Acknowledgments

The authors thank Drs. Robert Murphy and Karin Zemski Berry at the University of Colorado Lipidomics Core for conducting the hepatic PC analysis.

Funding

This work was supported by the National Institute of Environmental Health Sciences through Grants Nos. 1R01ES022697 and T32 ES007126.

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Authors and Affiliations

  1. Curriculum in Toxicology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA

    Madelyn C. Huang & Miroslav Stýblo

  2. Department of Nutrition, Gillings School of Global Public Health, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA

    Christelle C. Douillet & Miroslav Stýblo

Authors
  1. Madelyn C. Huang
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  2. Christelle C. Douillet
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  3. Miroslav Stýblo
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Corresponding author

Correspondence to Miroslav Stýblo.

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Huang, M.C., Douillet, C.C. & Stýblo, M. Knockout of arsenic (+3 oxidation state) methyltransferase results in sex-dependent changes in phosphatidylcholine metabolism in mice. Arch Toxicol 90, 3125–3128 (2016). https://doi.org/10.1007/s00204-016-1844-2

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  • DOI: https://doi.org/10.1007/s00204-016-1844-2

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