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l-Threonine and its analogue added to autoclaved solid medium suppress trichothecene production by Fusarium graminearum

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Abstract

Fusarium graminearum produces trichothecene mycotoxins under certain nutritional conditions. When l-Thr and its analogue l-allo-threonine were added to brown rice flour solid medium before inoculation, trichothecene production after 4 days of incubation was suppressed. A time-course analysis of gene expression demonstrated that l-Thr suppressed transcription of Tri6, a trichothecene master regulator gene, and a terpene cyclase Tri5 gene. Regulation of trichothecene biosynthesis by altering major primary metabolic processes may open up the possibility to develop safe chemicals for the reduction of mycotoxin contamination might be developed.

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Acknowledgements

This work was supported by Science and Technology Research Promotion Program for Agriculture, Forestry, Fisheries and Food Industry.

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    Authors and Affiliations

    1. Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8601, Japan

      Kazuyuki Maeda, Yuichi Nakajima, Yoshikazu Tanahashi, Yoshiyuki Kitou, Kyoko Kanamaru, Tetsuo Kobayashi & Makoto Kimura

    2. Division of Functional Genomics, Advanced Science Research Centre, Kanazawa University, 13-1 Takaramachi, Kanazawa, Ishikawa, 920-0934, Japan

      Akihiro Miwa & Takumi Nishiuchi

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    1. Kazuyuki Maeda
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    2. Yuichi Nakajima
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    Correspondence to Makoto Kimura.

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    Communicated by Erko Stackebrandt.

    Kazuyuki Maeda and Yuichi Nakajima contributed equally to this work.

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    Maeda, K., Nakajima, Y., Tanahashi, Y. et al. l-Threonine and its analogue added to autoclaved solid medium suppress trichothecene production by Fusarium graminearum . Arch Microbiol 199, 945–952 (2017). https://doi.org/10.1007/s00203-017-1364-3

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