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Isolation of an exopolysaccharide-producing heavy metal-resistant Halomonas sp. MG

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Abstract

An exopolysaccharide (EPS)-producing heavy metal-resistant Gram-negative bacterium was isolated from ore-contaminated soil. The selected strain was identified by 16S rDNA sequencing and designated as Halomonas sp. MG. Phylogenetic analysis of the gene sequence showed its close similarity with Halomonas sp. Field emission scanning electron microscopy analysis revealed that the EPS had a porous structure with small pores. X-ray diffractograms showed the non-crystalline nature of the EPS. Further, FTIR spectroscopic analysis revealed the presence of carboxyl, hydroxyl and amide groups corresponding to a typical EPS.

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References

  • Bafana A (2013) Diversity and metabolic potential of culturable root-associated bacteria from Origanum vulgare in sub-Himalayan region. World J Microb Biot 29:63–74

    Article  CAS  Google Scholar 

  • Bezawada J, Hoang NV, More TT, Yan S, Tyagi N, Surampalli RY (2013) Production of extracellular polymeric substances (EPS) by Serratia sp.1 using wastewater sludge as raw material and flocculation activity of the EPS produced. J Environ Manage 128:83–91

    Article  CAS  PubMed  Google Scholar 

  • Biswas J, Paul AK (2014) Production of extracellular polymeric substances by Halophilic bacteria of solar salterns. Chin J Biol. doi:10.1155/2014/205731

    Google Scholar 

  • Celik GY, Aslim B, Beyatli Y (2008) Characterization and production of the exopolysaccharide (EPS) from Pseudomonas aeruginosa G1 and Pseudomonas putida G12 strains. Carbohyd Polym 73:178–182

    Article  CAS  Google Scholar 

  • Hasan N, Gopal J, Wu HF (2011) Rapid, sensitive and direct analysis of exopolysaccharides from biofilm on aluminum surfaces exposed to sea water using MALDI-TOF MS. J Mass Spectrom 46:1160–1167

    Article  CAS  PubMed  Google Scholar 

  • Iqbal A, Bhatti HN, Nosheen S, Jamil A, Malik MA (2002) Histochemical and physicochemical study of bacterial exopolysaccharides. Biotechnology 1(1):28–33

    Article  Google Scholar 

  • Janaki V, Vijayaraghavan K, Ramasamy AK, Lee KJ, Oh BT, Kamala-Kannan S (2012) Competitive adsorption of Reactive Orange 16 and Reactive Brilliant Blue R on polyaniline/bacterial extracellular polysaccharides composite—A novel eco-friendly polymer. J Hazard Mater 241–242:110–117

    Article  PubMed  Google Scholar 

  • Kamala-Kannan S, Krishnamoorthy R (2006) Isolation of mercury resistant bacteria and influence of abiotic factors on bioavailability of mercury—a case study in Pulicat Lake north of Chennai, south east India. Sci Total Environ 367:341–353

    Article  Google Scholar 

  • Lordan S, Ross R, Stanton C (2011) Marine bioactives as functional food ingredients: potential to reduce the incidence of chronic diseases. Mar Drugs 9:1056–1100

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Luo J, Liu J, Ke C, Qiao D, Ye H, Sun Y, Zeng X (2009) Optimization of medium composition for the production of exopolysaccharides from Phellinus baumii Pilát in submerged culture and the immuno-stimulating activity of exopolysaccharides. Carbohyd Polym 78:409–415

    Article  CAS  Google Scholar 

  • Mittelman MW, Geesey GG (1985) Copper-binding characteristics of exopolymers from a freshwater-sediment bacterium. Appl Environ Microb 49:846–851

    CAS  Google Scholar 

  • Oh BT, Hur H, Lee KJ, Shanthi K, Soh BY, Lee WJ, Myung H, Kamala-Kannan S (2011) Suppression of Phytophthora blight on pepper (Capsicum annuum L.) by bacilli isolated from brackish environment. Biocontrol Sci Technol 21(11):1297–1311

    Article  Google Scholar 

  • Prasanna PHP, Bell A, Grandison AS, Charalampopoulos D (2012) Emulsifying, rheological and physicochemical properties of exopolysaccharide produced by Bifidobacterium longum subsp. infantis CCUG 52486 and Bifidobacterium infantis NCIMB 702205. Carbohyd Polym 90:533–540

    Article  CAS  Google Scholar 

  • Priester JH, Olson SG, Webb SM, Neu MP, Hersman LE, Holden PA (2006) Enhanced exopolymer production and chromium stabilization in Pseudomonas putida unsaturated biofilms. Appl Environ Microbiol 72:1988–1996

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Raza W, Yang W, Jun Y, Shakoor F, Huang Q, Shen Q (2012) Optimization and characterization of a polysaccharide produced by Pseudomonas fluorescens WR-1 and its antioxidant activity. Carbohyd Polym 90:921–929

    Article  CAS  Google Scholar 

  • Shao LI, Wub Z, Zhanga H, Chena W, Ai L, Guoa B (2014) Partial characterization and immunostimulatory activity of exopolysaccharides from Lactobacillus rhamnosus KF5. Carbohyd Polym 107:51–56

    Article  CAS  Google Scholar 

  • Song W, Ravindran V, Koel BE, Pirbazari M (2004) Nanofiltration of natural organic matter with H2O2/UV pretreatment: fouling mitigation and membrane surface characterization. J Membr Sci 241(1):143–160

    Article  CAS  Google Scholar 

  • Trabelsi I, Slima SB, Chaabane H, Riadh BS (2015) Purification and characterization of a novel exopolysaccharides produced by Lactobacillus sp. Int J Biol Macromol 74:541–546

    Article  CAS  PubMed  Google Scholar 

  • Wang J, Zhao X, Tian Z, Yang Y, Yang Z (2015) Characterization of an exopolysaccharide produced by Lactobacillus plantarum YW11 isolated from Tibet Kefir. Carbohyd Polym 125:16–25

    Article  CAS  Google Scholar 

  • Wu Q, Tun HM, Leung FCC, Shah NP (2014) Genomic insights into high exopolysaccharide-producing dairy starter bacterium Streptococcus thermophilus ASCC 1275. Sci Rep 4:4974

    PubMed Central  CAS  PubMed  Google Scholar 

  • Xu X, Li B, Kennedy JF, Xie BJ, Huang M (2007) Characterization of konjac glucomannan–gellan gum blend films and their suitability for release of nisin incorporated therein. Carbohyd Polym 70:192–197

    Article  CAS  Google Scholar 

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Acknowledgments

The research work was supported by the National Research Foundation of Korea (NRF) grant funded by the government (MEST; No. 2011-0020202). This research was also supported by Korea Ministry of Environment as “Eco-Innovation project (Project No. 2014000140003).”

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Correspondence to Byung-Taek Oh.

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Communicated by Erko Stackebrandt.

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Govarthanan, M., Shim, J., Praburaman, L. et al. Isolation of an exopolysaccharide-producing heavy metal-resistant Halomonas sp. MG. Arch Microbiol 198, 205–209 (2016). https://doi.org/10.1007/s00203-015-1173-5

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  • DOI: https://doi.org/10.1007/s00203-015-1173-5

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