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Flavobacterium maris sp. nov. isolated from shallow sediments of the Sea of Japan

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Abstract

An aerobic, gram-negative, yellow-pigmented, and non-motile bacterium designated KMM 9535T was isolated from a marine sediment sample obtained from the Sea of Japan seashore and subjected to a phylogenetic and phenotypic study. On the basis of 16S rRNA gene sequence analysis, strain KMM 9535T was placed to the genus Flavobacterium sharing the highest sequence similarities to Flavobacterium ahnfeltiae KCTC 32467T (99.3 %), Flavobacterium jumunjinense KCTC 23618T (96.5 %), Flavobacterium ponti KCTC 22802T (96.3 %), Flavobacterium urocaniciphilum JCM 19142T (96.1 %), and Flavobacterium gelidilacus LMG 21477T (95.8 %). The DNA–DNA hybridization value between strain KMM 9535T and the closest related F. ahnfeltiae KCTC 32467T was 33 %. Strain KMM 9535T grew at 5–36 °C and in the presence of 0–3 % (w/v) NaCl. It contained MK-6 as the predominant menaquinone, and the major fatty acids were iso-C15:0, iso-C17:1, iso-C15:1, and iso-C17:0 3-OH. The DNA G+C content was 28.8 mol%. On the basis of the results obtained, it is proposed strain KMM 9535T to be classified as a novel species of the genus Flavobacterium, Flavobacterium maris sp. nov., with the type strain of the species KMM 9535T (=NRIC 0920T = KCTC 42093T).

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Acknowledgments

We would like to thank Dr. Claudine Vereecke, BCCM™/LMG Bacteria Collection, Ghent University, Belgium, and Dr. Jung-Sook Lee, the Korean Collection for Type Cultures (KCTC), Biological Resource Center (BRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Korea, and Dr. Moriya Ohkuma, JCM, Japan Collection of Microorganisms, Japan, for providing Flavobacterium type strains for comparative analyses. This study was supported by a grant from the RSF “Biodiversity and biotechnological potential of marine bacteria and fungi” no. 14-14-00030.

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Authors and Affiliations

  1. G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, Prospect 100 Let Vladivostoku, 159, Vladivostok, 690022, Russia

    Lyudmila A. Romanenko, Valeriya V. Kurilenko & Valery V. Mikhailov

  2. NODAI Culture Collection Center, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-Ku, Tokyo, 156-8502, Japan

    Naoto Tanaka

  3. Institute of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, 690041, Russia

    Vassilii I. Svetashev

  4. Far Eastern Federal University, Oktyabr’skaya St., 27, Vladivostok, 690091, Russia

    Valery V. Mikhailov

Authors
  1. Lyudmila A. Romanenko
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  2. Naoto Tanaka
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  3. Vassilii I. Svetashev
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  4. Valeriya V. Kurilenko
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  5. Valery V. Mikhailov
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Corresponding author

Correspondence to Lyudmila A. Romanenko.

Additional information

Communicated by Erko Stackebrandt.

The DDBJ/GenBank/EMBL accession number of the 16S rRNA gene sequence of strain KMM 9535T is AB973429.

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Romanenko, L.A., Tanaka, N., Svetashev, V.I. et al. Flavobacterium maris sp. nov. isolated from shallow sediments of the Sea of Japan. Arch Microbiol 197, 941–947 (2015). https://doi.org/10.1007/s00203-015-1128-x

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  • DOI: https://doi.org/10.1007/s00203-015-1128-x

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