Abstract
A strictly anaerobic bacterial strain (WN037T) was isolated from a methanogenic reactor. Cells were Gram-positive rods. Strain WN037T was asaccharolytic. The strain fermented l-lysine in the presence of B-vitamin mixture or vitamin B12 and produced acetate and butyrate. l-arginine and casamino acids poorly supported the growth. Strain WN037T used neither other amino acids nor organic acids examined. The strain had C18:1 ω7c, C16:0 and C18:1 ω7c DMA as the predominant cellular fatty acids. The genomic DNA G + C content was 44.2 mol %. Phylogenetic analysis based on the 16S rRNA gene sequence placed strain WN037T in the family Eubacteriaceae in the class Clostridia. The closest relative was Eubacterium pyruvativorans (sequence similarity, 92.8 %). Based on the comprehensive analyses, the novel genus and species, Aminocella lysinolytica gen. nov., sp. nov. was proposed to accommodate the strain. The type strain is WN037T (= JCM 19863T = DSM 28287T).
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This work was partly supported by a grant-in-aid from the Institute for Fermentation, Osaka.
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The authors declare that they have no conflict of interest.
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Communicated by Erko Stackebrandt.
The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain WN037T is AB298740.
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Ueki, A., Shibuya, T., Kaku, N. et al. Aminocella lysinolytica gen. nov., sp. nov., a l-lysine-degrading, strictly anaerobic bacterium in the class Clostridia isolated from a methanogenic reactor of cattle farms. Arch Microbiol 197, 97–104 (2015). https://doi.org/10.1007/s00203-014-1066-z
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DOI: https://doi.org/10.1007/s00203-014-1066-z