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Jilinibacillus soli gen. nov., sp. nov., a novel member of the family Bacillaceae

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Abstract

A Gram-positive, aerobic, rod-shaped, motile, endospore-forming bacterium, designated strain A12T, was isolated from a saline and alkali soil samples in Baicheng City, western of Jilin Province, China. Growth occurred in 15–45 °C (optimum, 30 °C) and at pH 7.0–11.5 (optimum, pH 9.0) and in the presence of 0–10 % (w/v) NaCl [optimum, 1–3 % (w/v) NaCl]. Meso-DAP was present in the peptidoglycan. The predominant menaquinone was MK-7. The major polar lipid profile was phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidyl inositol-methyl and phosphotidylinositol dimannosid. The major fatty acid (>10 % of total fatty acids) was anteiso-C15:0. DNA G + C content was 36.2 mol %. The level of 16S rRNA gene sequence similarity between strain A12T and other recognized species of the family was below 95.6 %. Phylogenetic analysis based on 16S rRNA gene sequence data indicated that the strain A12T fell with the family Bacillaceae and formed a distinct taxon. Based on physiological, chemotaxonomic and phylogenetic analyses, strain A12T was considered to represent a novel species of a new genus, for which the name Jilinibacillus soli gen. nov., sp. nov. was proposed. The type strain of Jilinibacillus soli was A12T (=GIMN1.014T = CCTCC M2011164T = KCTC 33417T).

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Acknowledgments

We thank Prof. H.H. Zhu (Guangdong Provincial Microbial Culture Collection and Application Key Laboratory, Guangdong Institute of Microbiology) for transmission electron microscopy and phylogenetic analysis. This work was supported by Chinese Universities Scientific Fund (QN2013035 and 2014YB044), Natural Science Basic Research Plan in Shaanxi Province of China (2013JQ3006), Specialized Research Fund for the Doctoral Program of Higher Education (20130204120018 and 20130204120038), Agromicrobiology Research and Utilization Innovative Research Team in Jilin Province (20121812), National Natural Science Foundation of China (31401839).

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Authors and Affiliations

  1. College of Life Sciences, Northwest A&F University, Yangling, 712100, People’s Republic of China

    Jingying Liu & Pengda Ma

  2. Institute of Plant Protection, Jilin Academy of Agricultural Sciences, Gongzhuling, 136100, People’s Republic of China

    Qian Du & Qiyun Li

  3. College of Life Sciences, Jilin Agricultural University, Changchun, 130118, People’s Republic of China

    Xiuran Wang

  4. Chemical and Pharmaceutical Engineering Department, Southeast University Chengxian College, Nanjing, 210088, People’s Republic of China

    Meina Li

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  1. Jingying Liu
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  2. Xiuran Wang
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  3. Meina Li
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  4. Qian Du
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  5. Qiyun Li
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Corresponding authors

Correspondence to Qiyun Li or Pengda Ma.

Additional information

Communicated by Erko Stackebrandt.

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203_2014_1032_MOESM1_ESM.jpg

Supplementary Fig. S1 A maximum-likelihood phylogenetic tree based on 16S rRNA gene sequences of strain A12T and related taxa. Bootstrap values are shown in percentages of 1,000 replicates, when more than 50 %. Bar, 0.01 substitutions per nucleotide position. (JPEG 449 kb)

203_2014_1032_MOESM2_ESM.jpg

Supplementary Fig. S2 A maximum-parsimony phylogenetic tree based on 16S rRNA gene sequences of strain A12T and related taxa. Bootstrap values are shown in percentages of 1,000 replicates, when more than 50 %. Bar, 0.01 substitutions per nucleotide position. (JPEG 461 kb)

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Liu, J., Wang, X., Li, M. et al. Jilinibacillus soli gen. nov., sp. nov., a novel member of the family Bacillaceae . Arch Microbiol 197, 11–16 (2015). https://doi.org/10.1007/s00203-014-1032-9

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  • DOI: https://doi.org/10.1007/s00203-014-1032-9

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