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Virulence determinants and biofilm production among Trueperella pyogenes recovered from abscesses of captive forest musk deer

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Abstract

Trueperella pyogenes (formerly Arcanobacterium) is commonly isolated from domesticated or wild ruminants as an opportunistic pathogen. To investigate the role of virulence determinants (VDs) and biofilm production in T. pyogenes isolates, a total of 36 T. pyogenes were collected from abscesses of forest musk deer in Miyaluo Farm (Sichuan Province, China). The prevalence of VDs and associations with clonal types, antibiotic resistance and biofilm production were analyzed by PCR and bioassay. Finally, T. pyogenes isolates were separated into three clonal types based on the DNA fingerprinting of BOX-PCR. Isolates with less VDs obtained from sick forest musk deer were mainly belonged to Type 1, and the isolates with robust VD repertoire obtained from dead forest musk deer were included in Type 3. Accordingly, resistant isolates exhibited significant lower virulence than susceptible ones. Majority of T. pyogenes isolates of this study were capable of producing a biofilm. However, no VDs presence and antibiotic resistance were statistically associated with biofilm production. In conclusion, the current study demonstrated that T. pyogenes was probably the primary pathogen of abscesses in the forest musk deer. Moreover, as an animal origin pathogen, the increasing resistance of T. pyogenes isolates could also associate with a decreased virulence.

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Acknowledgments

This work was supported by the National Basic Research Program of China (973 Project: 2012CB722207) and National Forestry Bureau of China (2130211). The authors would like to acknowledge Xuxin Li from Miyaluo Farm for collecting the samples. We thank Kiswendsida Ouedeaogo and the anonymous reviewers for editing the manuscript.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Sichuan Key Laboratory of Conservation Biology on Endangered Wildlife, College of Life Science, Sichuan University, No. 24, South Section 1, Yihuan Road, Chengdu, 610064, Sichuan, People’s Republic of China

    Kelei Zhao & Xiuyue Zhang

  2. Laboratory of Chemical Biology, College of Chemical Engineering, Sichuan University, Chengdu, 610064, People’s Republic of China

    Yongqiang Tian

  3. Key Laboratory of Bioresources and Ecoenvironment (Ministry of Education), College of Life Science, Sichuan University, Chengdu, 610064, People’s Republic of China

    Bisong Yue & Hongning Wang

Authors
  1. Kelei Zhao
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  2. Yongqiang Tian
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  3. Bisong Yue
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  4. Hongning Wang
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  5. Xiuyue Zhang
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Corresponding author

Correspondence to Xiuyue Zhang.

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Communicated by Jorge Membrillo-Hernández.

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Zhao, K., Tian, Y., Yue, B. et al. Virulence determinants and biofilm production among Trueperella pyogenes recovered from abscesses of captive forest musk deer. Arch Microbiol 195, 203–209 (2013). https://doi.org/10.1007/s00203-013-0869-7

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  • DOI: https://doi.org/10.1007/s00203-013-0869-7

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