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γ-Butyrolactone autoregulators and receptor proteins in non-Streptomyces actinomycetes producing commercially important secondary metabolites

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Abstract.

The presence of γ-butyrolactone autoregulators and their receptor proteins were investigated in five representative strains of non-Streptomyces actinomycetes producing commercially important secondary metabolites. Ethyl acetate extracts of culture were assayed using wild-type S. virginiae for virginiae butanolide, S. lavendulae FRI-5 for IM-2, and S. griseus HH1 for A-factor. Actinoplanes teichomyceticus and Amycolatopsis mediterranei were shown to produce autoregulators. Corresponding autoregulator-binding activities were found in the crude cell-free lysates of these strains, using the binding assay with tritium-labeled autoregulator analogues as ligands, which suggests that non-Streptomyces actinomycetes might have autoregulator-dependent signaling cascades.

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Fig. 1A, B.

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Acknowledgements

This work was supported in part by grant from the Korea Research Foundation (KRF-2002-042-C00086).

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Authors and Affiliations

  1. International Center for Biotechnology, Osaka University, 2-1 Yamadaoka, Suita, 565-0871, Osaka, Japan

    Sun-Uk Choi, Hiroshi Kinosita & Takuya Nihira

  2. PrimBioR&Ds, Institute for Genome Reconstruction, Kyungnam University, 449 Wolyong-dong, 631-701, Masan, Republic of Korea

    Chang-Kwon Lee

  3. Department of Food Science and Biotechnology, Division of Life Science, Kyungnam University, 449 Wolyong-dong, 631-701, Masan, Republic of Korea

    Yong-Il Hwang

Authors
  1. Sun-Uk Choi
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  2. Chang-Kwon Lee
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  3. Yong-Il Hwang
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  4. Hiroshi Kinosita
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  5. Takuya Nihira
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Correspondence to Takuya Nihira.

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Choi, SU., Lee, CK., Hwang, YI. et al. γ-Butyrolactone autoregulators and receptor proteins in non-Streptomyces actinomycetes producing commercially important secondary metabolites. Arch Microbiol 180, 303–307 (2003). https://doi.org/10.1007/s00203-003-0591-y

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  • DOI: https://doi.org/10.1007/s00203-003-0591-y

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