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Use of the Integrated Biomarker Response to Measure the Effect of Short-term Exposure to Dibenz[a,h]anthracene in Common carp (Cyprinus carpio)

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Abstract

Dibenz[a,h]anthracene (DbA) is a polycyclic aromatic hydrocarbon that is released into the environment through incomplete combustion of gasoline, cigarettes, and coal tar. The effects of short-term (10 days) exposure of common carp (Cyprinus carpio) to DbA (0–50 µg L−1) were evaluated using the following four biomarkers: DNA damage, 7-ethoxyresorufin-O-deethylase (EROD) activity, acetylcholinesterase (AChE) activity, and vitellogenin (VTG) levels. An integrated biomarker response (IBR) was calculated for exposure to DbA, and the results were compared with those in our previous study of two other PAHs, benzo[k]fluoranthene (BkF) and benzo[a]pyrene (BaP). DbA exposure resulted in a significant (p < 0.05) increase in DNA damage, EROD activity, and VTG levels relative to the control. By contrast, DbA did not affect AChE activity. The IBR increased as the concentration of DbA increased. Based on the IBR values, the order of toxicity for the PAHs was BkF > BaP > DbA. Our results suggest that the IBR can be used as a quantitative tool for evaluating the responses of multiple biomarkers to PAH exposure.

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References

  • Aas E, Baussant T, Balk L, Liewenborg B, Andersen OK (2000) PAH metabolites in bile, cytochrome P4501A and DNA adducts as environmental risk parameters for chronic oil exposure: a laboratory experiment with Atlantic cod. Aquat Toxicol 51:241–258

    Article  CAS  Google Scholar 

  • Akcha F, Izuel C, Venier P, Budzinski H, Burgeot T, Narbonne J-F (2000) Enzymatic biomarker measurement and study of DNA adduct formation in benzo[a]pyrene-contaminated mussels, Mytilus galloprovincialis. Aquat Toxicol 49:269–287

    Article  CAS  Google Scholar 

  • Balam M, Arnulfo A (2011) DNA damage caused by polycyclic aromatic hydrocarbons: mechanisms and markers. In: Clark C (ed) Selected topics in DNA repair ISBN: 978-953-307-606-5, InTech, doi: 10.5772/22527

  • Beliaeff B, Burgeot T (2002) Integrated biomarker response: a useful tool for ecological risk assessment. Environ Toxicol Chem 21:1316–1322

    Article  CAS  Google Scholar 

  • Bols NC, Schirmer K, Joyce EM, Dixon DG, Greenberg BM, Whyte JJ (1999) Ability of polycyclic aromatic hydrocarbons to induce 7-ethoxyresorufin-o-deethylase activity in a trout liver cell line. Ecotox Environ Safe 44:118–128

    Article  CAS  Google Scholar 

  • Chang RM, Wood AW, Huang MT, Xie JG, Cui XX, Reuhl KR, Boyd DR, Lin Y, Shih WJ, Balani SK, Yagi H, Jerina DM, Conney AH (2013) Mutagenicity and tumorigenicity of the four enantiopure bay-region 3,4-diol-1,2-epoxide isomers of dibenz[a, h]anthracene. Carcinogenesis 34:2184–2191

    Article  CAS  Google Scholar 

  • Clemons JH, Allan LM, Marvin CH, Wu Z, Mccarry BE, Bryant DW (1998) Evidence of estrogen- and TCDD-like activities in crude and fractionated extracts of PM10 air particulate material using in vitro gene expression assay. Environ Sci Technol 32:1853–1860

    Article  CAS  Google Scholar 

  • Crago J, Corsi SR, Weber D, Bannerman R, Klaper R (2011) Linking biomarkers to reproductive success of caged fathead minnows in streams with increasing urbanization. Chemosphere 82:1669–1674

    Article  CAS  Google Scholar 

  • Gestel CAM, Brummelen TC (1996) Incorporation of the biomarkers concept in ecotoxicology calls for a redefinition of terms. Ecotoxicology 5:217–225

    Article  Google Scholar 

  • Gravato C, Santos MA (2002) Juvenile sea bass liver P450, EROD induction, and erythrocytic genotoxic responses to PAH and PAH-like compounds. Ecotoxicol Environ Saf 51:115–127

    Article  CAS  Google Scholar 

  • Irwin RJ, VanMouwerik M, Stevens L, Seese MD, Basham W (1997) Environmental contaminants encyclopedia. National Park Service—Water Resources Division, Fort Collins

  • Jung JH, Kim SJ, Lee TK, Shim WJ, Woo SN, Kim DJ, Han CH (2008) Biomarker responses in caged rockfish (Sebastes schlegeli) from Masan bay and Haegeumgang, South Korea. Mar Pollut Bull 57:599–606

    Article  CAS  Google Scholar 

  • Jung JH, Kim MK, Yim UH, Ha SY, An JG, Won JH, Han GM, Kim NS, Addison RF, Shim WJ (2011) Biomarker responses in pelagic and benthic fish over 1 year following the Hebei Spirit oil spill (Taean, Korea). Mar Pollut Bull 62:1859–1866

    Article  CAS  Google Scholar 

  • Kennedy SW, Jones SP (1994) Simultaneous measurement of cytochrome P4501A catalytic activity and total protein concentration with a fluorescence plate reader. Anal Biochem 222:217–223

    Article  CAS  Google Scholar 

  • Kim WK, Kim JH, Yeom DH, Lee SK (2008a) Effect evaluation of Benzo[a]pyrene on multiple biomarkers in Common carp (Cyprinus carpio). J Environ Toxicol 23:171–178

    Google Scholar 

  • Kim WK, Kim JH, Yeom DH, Lee SK (2008b) Responses of various biomarkers in Common carp (Cyprinus carpio) exposed to Benzo[k]fluoranthene. Kor J Limnol 41:331–337

    Google Scholar 

  • Kim JH, Yeom DH, Kim JH, An KG (2012) New ecological health assessment approaches of an urban stream using molecular and physiological level biomarkers and bioindicators. Anim Cells Syst 16:329–336

    Article  Google Scholar 

  • Kirby MF, Smith AJ, Rooke J, Neall P, Scott AP, Katsiadaki I (2007) Ethoxyresorufin-O-deethylase (EROD) and vitellogenin (VTG) in flounder (Platichthys flesus): system interaction, crosstalk and implications for monitoring. Aquat Toxicol 81:233–244

    Article  CAS  Google Scholar 

  • Lam PKS, Gray JS (2003) The use of biomarkers in environmental monitoring programmes. Mar Pollut Bull 46:182–186

    Article  CAS  Google Scholar 

  • OECD (1992) Test No. 203: Fish, acute toxicity test, OECD guidelines for the testing of chemicals, section 2. OECD Publishing, Paris

  • OECD (2001) Screening information data set of OECD High production volume chemicals program: dimethylformamide. OECD Publishing, Paris

  • Roche H, Buet A, Ramade F (2002) Accumulation of lipophilic microcontaminants and biochemical responses in Eels from the Camargue Biosphere Reserve. Ecotoxicology 11:155–164

    Article  CAS  Google Scholar 

  • Serafim A, Company R, Lopes B, Fonseca VF, França S, Vasconcelos RP (2012) Application of an integrated biomarker response index (IBR) to assess temporal variation of environmental quality in two Portuguese aquatic systems. Ecol Indic 19:215–225

    Article  CAS  Google Scholar 

  • Tyler CR, Van der Eerden B, Jobling S, Panter G, Sumpter JP (1996) Measurement of vitellogenin, a biomarker for exposure to oestrogenic chemicals, in a wide variety of cyprinid fish. J Comp Physiol B 166:418–426

    Article  CAS  Google Scholar 

  • van der Oost R, Beyer J, Vermeulen NPE (2003) Fish bioaccumulation and biomarkers in environmental risk assessment: a review. Environ Toxicol Pharm 13:57–149

    Article  Google Scholar 

  • van Schanke A, Boon JP, Aardoom Y, van Leest A, van Schooten FJ, Maas L, van den Berg M, Everaarts JM (2000) Effect of a dioxin-like PCB (CB 126) on the biotransformation and genotoxicity of benzo[a]pyrene in the marine flatfish dab (Limanda limanda). Aquat Toxicol 50:403–415

    Article  Google Scholar 

  • Varanasi U, Reichert WL, Stein JE (1989) 32P-postlabeling analysis of DNA adducts in liver of wild English sole (Parophrys vetulus) and winter flounder (Pseudopleuronectes americanus). Cancer Res 49:1171–1177

    CAS  Google Scholar 

  • Zakaria MP, Takaka H, Tsutsumi S, Ohno K, Yamaka J, Kouno E, Kumata H (2002) Distribution of polycyclic aromatic hydrocarbons (PAHs) in Rivers and Estuaries in Malaysia: a widespread input of petrogenic PAHs. Environ Sci Technol 26:1907–1918

    Article  Google Scholar 

  • Zhang ZL, Hong HS, Zhou JL, Yu G (2004) Phase association of polycyclic aromatic hydrocarbons in the Minjiang River Estuary, China. Sci Total Environ 323:71–86

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This study was supported by funding from the Korea Institute of Toxicology (Korea).

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Authors and Affiliations

  1. Environmental Biology and Chemistry Research Center, Korea Institute of Toxicology, Jinju, 660-844, Korea

    Ja-Hyun Kim

  2. System Toxicology Research Center, Korea Institute of Toxicology, Daejeon, 305-343, Korea

    Woo-Keun Kim

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  1. Ja-Hyun Kim
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Correspondence to Woo-Keun Kim.

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Kim, JH., Kim, WK. Use of the Integrated Biomarker Response to Measure the Effect of Short-term Exposure to Dibenz[a,h]anthracene in Common carp (Cyprinus carpio). Bull Environ Contam Toxicol 96, 496–501 (2016). https://doi.org/10.1007/s00128-015-1726-y

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  • DOI: https://doi.org/10.1007/s00128-015-1726-y

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