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Effects of Cadmium and Copper Biosorption on Chlorella vulgaris

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Abstract

Changes in protein levels and lipid compositions in algal cells indicate the severity of stress related to toxic concentrations of heavy metals. In this study, the effects of exposure to cadmium and copper on Chlorella vulgaris and its capacity to remove metals were evaluated. The data revealed ion removal activity by microalgae under all treatments and different levels of protein expression after 48 h of exposure. Furthermore, we analyzed lipids contents to characterize them.

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References

  • Arunakumara KKIU, Zhang X (2008) Heavy metal bioaccumulation and toxicity with special reference to microalgae. J Ocean Univ Chin 7:60–64

    Article  CAS  Google Scholar 

  • Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917

    Article  CAS  Google Scholar 

  • Corrêa SC et al (2011) Evaluation of dehydrated marolo (Annona crassiflora) flour and carpels by freeze-drying and convective hot-air drying. Food Res Int 44:2385–2390

    Article  Google Scholar 

  • Ercal N, Gurer-Orhan H, Aykin-Burns N (2001) Toxic metals and oxidative stress part I: mechanisms involved in metal induced oxidative damage. Curr Top Med Chem 6:529–539

    Article  Google Scholar 

  • Ferreira CR et al (2010) Single embryo and oocyte lipid fingerprinting by mass spectrometry. J Lipid Res 51:1218–1227

    Article  CAS  Google Scholar 

  • Garcia JS et al (2009) Evaluation of metal-ion stress in sunflower (Helianthus annuus L.) leaves through proteomic changes. Metallomics 1:107–113

    Article  CAS  Google Scholar 

  • He H, Rodgers RP, Marshall AG, Hsu CS (2011) Algae polar lipids characterized by online liquid chromatography coupled with hybrid linear quadrupole ion Trap/Fourier transform ion cyclotron resonance mass spectrometry. Energy Fuels 25:4770–4775

    Article  CAS  Google Scholar 

  • Khummongkol D, Canterford G, Fryer C (1982) Accumulation of heavy metals in unicellular algae. Biotechnol Bioeng 24:2646–2660

    Article  Google Scholar 

  • Lei A, Hu Z, Wong Y, Tam NF (2007) Removal of Xuoranthene and pyrene by different microalgal species. Bioresour Technol 98:273–278

    Article  CAS  Google Scholar 

  • Monteiro MC, Castro PML, Malcata FX (2011) Capacity of simultaneous removal of zinc and cadmium from contaminated media, by two microalgae isolated from a polluted site. Environ Chem Lett 9:511–517

    Article  CAS  Google Scholar 

  • Quian H, Li J, Sun L, Chen W, Sheng GD, Liu W, Fu Z (2009) Combined effect of copper and cadmium on Chlorella vulgaris growth and photosynthesis-related gene transcription. Aquat Toxicol 94:56–61

    Article  Google Scholar 

  • Regaldo et al (2013) Bioaccumulation and toxicity of copper and lead in Chlorella vulgaris. J Algal Biomass Utln 4:59–66

    Google Scholar 

  • Ruangsomboon S, Wongrat L (2006) Bioaccumulation of cadmium in an experimental aquatic food chain involving phytoplankton (Chlorella vulgaris), zooplankton (Moina macrocopa), and the predatory catfish Clarias macrocephalus × C. Gariepinus. Aquat Toxicol 78:15–20

    Article  CAS  Google Scholar 

  • Sabatini SE et al (2009) Oxidative stress and antioxidant defenses in two green microalgae exposed to copper. Ecotox Environ Safe 72:1200–1206

    Article  CAS  Google Scholar 

  • Simmons DBD, Hayward AR, Hutchinson TC, Emery RJN (2009) Identification and quantification of glutathione and phytochelatins from C. vulgaris by RP-HPLC ESI-MS/MS and oxygen-free extraction. Anal Bional Chem 395:809–817

    Article  CAS  Google Scholar 

  • Zheng H, Yin J, Gao Z, Huang H, Ji X, Dou C (2011) Disruption of Chlorella vulgaris cells for the release of biodiesel-producing lipids: a comparison of grinding, ultrasonication, bead milling, enzymatic lysis, and microwaves. Appl Biochem Biotechnol 164:1215–1224

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors acknowledge to CNPq, CAPES (Process Casadinho/PROCAD 552387/2011-8) and FAPEMIG (Process APQ-00410-09) for financial support and Prof. Pio Colepicollo (University of São Paulo, Brazil) for providing microalgae culture.

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Correspondence to Jerusa S. Garcia.

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de Abreu, F.C.P., da Costa, P.N.M., Brondi, A.M. et al. Effects of Cadmium and Copper Biosorption on Chlorella vulgaris . Bull Environ Contam Toxicol 93, 405–409 (2014). https://doi.org/10.1007/s00128-014-1363-x

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  • DOI: https://doi.org/10.1007/s00128-014-1363-x

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