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Enantioselective Effects of Methamidophos on the Coelomocytes Lysosomal Membrane Stability of Eisenia fetida

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Abstract

Many of organophosphorous insecticides are chiral compounds. In this study, the enantioselective effects of organophosphate insecticide methamidophos on the coelomocytes lysosomal membrane stability of earthworm Eisenia fetida were studied: (1) The enantiomers of methamidophos were absolutely separated by high-performance liquid chromatography with a commercial chiral column; (2) The neutral red retention assay was used to judge the lysosomal membrane stability. The results showed that with the concentration increasing, lysosomal membranes have been significantly destroyed by individual stereoisomers and racemate of methamidophos. The neutral red retention times were significantly descended from 76.88 to 29.78 min. Both (+)- and (−)-methamidophos showed more prone to destroy the integrity of the lysosomal membrane than the racemate. However, the different effect between stereoisomers is slight.

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Acknowledgments

This study was financially supported by the Natural Science Foundation of Zhejiang Province, China (Y5100253), and the National Natural Science Foundation of China (No. 21007058).

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Authors and Affiliations

  1. Research Center of Environmental Science, Zhejiang University of Technology, Hangzhou, 310032, People’s Republic of China

    Linhua Chen & Yun Ma

  2. Institute of Environmental Science and Engineering, Hangzhou Dianzi University, Hangzhou, 310018, People’s Republic of China

    Xianting Lu

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  1. Linhua Chen
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  2. Xianting Lu
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  3. Yun Ma
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Correspondence to Yun Ma.

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Chen, L., Lu, X. & Ma, Y. Enantioselective Effects of Methamidophos on the Coelomocytes Lysosomal Membrane Stability of Eisenia fetida . Bull Environ Contam Toxicol 89, 1161–1164 (2012). https://doi.org/10.1007/s00128-012-0861-y

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  • DOI: https://doi.org/10.1007/s00128-012-0861-y

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