Original Article

Theoretical and Applied Genetics

, Volume 104, Issue 1, pp 61-66

Evaluating the potential of SSR flanking regions for examining taxonomic relationships in the Vitaceae

  • M. RossettoAffiliated withCentre for Plant Conservation Genetics, Southern Cross University, PO Box 157 Lismore NSW, Australia 2480 e-mail: mrossett@scu.edu.au Tel.: +61-2-66203458, Fax: +61-2-66222080
  • , J. McNallyAffiliated withCentre for Plant Conservation Genetics, Southern Cross University, PO Box 157 Lismore NSW, Australia 2480 e-mail: mrossett@scu.edu.au Tel.: +61-2-66203458, Fax: +61-2-66222080
  • , R. J. HenryAffiliated withCentre for Plant Conservation Genetics, Southern Cross University, PO Box 157 Lismore NSW, Australia 2480 e-mail: mrossett@scu.edu.au Tel.: +61-2-66203458, Fax: +61-2-66222080

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Abstract  

Three EST-derived microsatellite loci from Vitis vinifera were amplified and sequenced across eight species of Vitaceae from four different genera. Phylogenetic analysis of the microsatellite’s flanking regions produced informative results in congruence with previous studies. Generic relationships were respected and the data produced sufficient inter-specific variation to distinguish between Cayratia acris and Cayratia saponaria, two very closely related species. Overall, the sequence alignments showed that priming sites were conserved, whereas microsatellite repeats were present in most cases but structurally variable. The sequence data provided information on the evolutionary patterns of various microsatellite repeats and their correlation to evolutionary relationships among taxa.

Keywords Microsatellites Vitis Mutation Flanking regions ESTs