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Mapping quantitative trait loci for root development under hypoxia conditions in soybean (Glycine max L. Merr.)

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Abstract

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Greatest potential, QTLs for hypoxia and waterlogging tolerance in soybean roots were detected using a new phenotypic evaluation method.

Abstract

Waterlogging is a major environmental stress limiting soybean yield in wet parts of the world. Root development is an important indicator of hypoxia tolerance in soybean. However, little is known about the genetic control of root development under hypoxia. This study was conducted to identify quantitative trait loci (QTLs) responsible for root development under hypoxia. Recombinant inbred lines (RILs) developed from a cross between a hypoxia-sensitive cultivar, Tachinagaha, and a tolerant landrace, Iyodaizu, were used. Seedlings were subjected to hypoxia, and root development was evaluated with the value change in root traits between after and before treatments. We found 230 polymorphic markers spanning 2519.2 cM distributed on all 20 chromosomes (Chrs.). Using these, we found 11 QTLs for root length (RL), root length development (RLD), root surface area (RSA), root surface area development (RSAD), root diameter (RD), and change in average root diameter (CARD) on Chrs. 11, 12, 13 and 14, and 7 QTLs for hypoxia tolerance of these root traits. These included QTLs for RLD and RSAD between markers Satt052 and Satt302 on Chr. 12, which are important markers of hypoxia tolerance in soybean; those QTLs were stable between 2 years. To validate the QTLs, we developed a near-isogenic line with the QTL region derived from Iyodaizu. The line performed well under both hypoxia and waterlogging, suggesting that the region contains one or more genes with large effects on root development. These findings may be useful for fine mapping and positional cloning of gene responsible for root development under hypoxia.

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Abbreviations

CARD:

Change in average root diameter

Chr.:

Chromosome

DAS:

Days after sowing

HTI:

Hypoxia tolerance index

LG:

Linkage group

NIL:

Near-isogenic line

QTL:

Quantitative trait loci

RD:

Root diameter

RDW:

Root dry weight

RIL:

Recombinant inbred lines

RL:

Root length

RLD:

Root length development

RSA:

Root surface area

RSAD:

Root surface area development

SDW:

Shoot dry weight

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Acknowledgements

This work was supported by a grant from the Ministry of Agriculture, Forestry and Fisheries of Japan (Genomics-based Technology for Agricultural Improvement, SFC-1005).

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Authors and Affiliations

  1. Graduate School of Bioresource and Environmental Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka, 812-8581, Japan

    Loc Van Nguyen, Keisuke Suematsu, Tomomi Abiko & Toshihiro Mochizuki

  2. NARO Institute of Crop Science, 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8518, Japan

    Ryoji Takahashi, Takasi Sayama & Masao Ishimoto

  3. Jomo Kenyatta University of Agriculture and Technology, P.O. Box 62000-00200, Nairobi, Kenya

    Stephen Mwangi Githiri

  4. Graduate School of Life and Environmental Sciences, University of Tsukuba, 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8518, Japan

    Tito O. Rodriguez

  5. University Farm, Kyushu University, 111 Harumachi, Kasuya-cho, Kasuya-gun, Fukuoka, 811-2307, Japan

    Nobuko Tsutsumi & Sayuri Kajihara

  6. Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka, 565-0871, Japan

    Kyuya Harada

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  1. Loc Van Nguyen
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Correspondence to Loc Van Nguyen.

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Communicated by Brian Diers.

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Van Nguyen, L., Takahashi, R., Githiri, S.M. et al. Mapping quantitative trait loci for root development under hypoxia conditions in soybean (Glycine max L. Merr.). Theor Appl Genet 130, 743–755 (2017). https://doi.org/10.1007/s00122-016-2847-3

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