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Genetic mapping with an inbred line-derived F2 population in potato

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This is the first report of the production and use of a diploid inbred line-based F2 population for genetic mapping in potato.

Abstract

Potato (Solanum tuberosum L.) is an important global food crop, for which tetrasomic inheritance and self-incompatibility have limited both genetic discovery and breeding gains. We report here on the creation of the first diploid inbred line-derived F2 population in potato, and demonstrate its utility for genetic mapping. To create the population, the doubled monoploid potato DM1-3 was crossed as a female to M6, an S7 inbred line derived from the wild relative S. chacoense, and a single F1 plant was then self-pollinated. A genetic linkage map with 2264 single nucleotide polymorphisms was constructed and used to improve the physical anchoring of superscaffolds in the potato reference genome, which is based on DM1-3. Segregation was observed for skin and flesh color, skin and flesh pigment intensity, tuber shape, anther development, jelly end, and the presence of eye tubers instead of normal sprouts. Using the R/qtl software, we detected 10 genes, 7 of which have been previously mapped and 3 for which this is the first publication. The latter category includes tightly linked genes for the jelly end and eye tuber traits on chromosome 5. The development of recombinant inbred lines from this F2 population by single-seed descent is underway and should facilitate even better resolution of these and other loci.

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Acknowledgments

The authors acknowledge the contributions made by David Douches (Michigan State University), who provided SNP data; Grace Christensen, who extracted DNA for SNP genotyping; Andy Hamernik, who was responsible for the greenhouse and field trials; and Xiaoxi Liu (Ohio State University), who scored tuber shape in 2014. This project was supported by USDA NIFA 2014-67013-22434.

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Authors and Affiliations

  1. Department of Horticulture, University of Wisconsin, Madison, WI, 53706, USA

    Jeffrey B. Endelman & Shelley H. Jansky

  2. USDA Agricultural Research Service, Madison, WI, 53706, USA

    Shelley H. Jansky

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  1. Jeffrey B. Endelman
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  2. Shelley H. Jansky
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Correspondence to Shelley H. Jansky.

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The authors declare they have no conflict of interest.

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Communicated by H. J. van Eck.

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Endelman, J.B., Jansky, S.H. Genetic mapping with an inbred line-derived F2 population in potato. Theor Appl Genet 129, 935–943 (2016). https://doi.org/10.1007/s00122-016-2673-7

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