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Influence of day-length and isolates of Phytophthora infestans on field resistance to late blight of potato

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Abstract

Main and interaction effects of day-length and pathogen isolate on the reaction and expression of field resistance to Phytophthora infestans were analyzed in a sample of standard clones for partial resistance to potato late blight, and in the BCT mapping population derived from a backcross of Solanum berthaultii to Solanum tuberosum. Detached leaves from plants grown in field plots exposed to short- and long day-length conditions were independently inoculated with two P. infestans isolates and incubated in chambers under short- and long photoperiods, respectively. Lesion growth rate (LGR) was used for resistance assessment. Analysis of variance revealed a significant contribution of genotype × isolate × day-length interaction to variation in LGR indicating that field resistance of genotypes to foliar late blight under a given day-length depended on the infecting isolate. An allele segregating from S. berthaultii with opposite effects on foliar resistance to late blight under long- and short day-lengths, respectively, was identified at a quantitative trait locus (QTL) that mapped on chromosome 1. This allele was associated with positive (decreased resistance) and negative (increased resistance) additive effects on LGR, under short- and long day-length conditions, respectively. Disease progress on whole plants inoculated with the same isolate under field conditions validated the direction of its effect in short day-length regimes. The present study suggests the occurrence of an isolate-specific QTL that displays interaction with isolate behavior under contrasting environments, such as those with different day-lengths. This study highlights the importance of exposing genotypes to a highly variable population of the pathogen under contrasting environments when stability to late blight resistance is to be assessed or marker-assisted selection is attempted for the manipulation of quantitative resistance to late blight.

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Acknowledgments

The authors appreciate the technical assistance and support of W. Pérez and E. de la Torre in inoculum preparation and infection bioassays. They also thank F. Mendiburu for statistical analysis support, and J. Huaccachi and A. Gómez for assistance during the experimental work

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Authors and Affiliations

  1. International Potato Center, Lima 12, P.O. Box 1558, Lima, Peru

    E. Mihovilovich, S. Munive & M. Bonierbale

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  1. E. Mihovilovich
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  2. S. Munive
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  3. M. Bonierbale
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Correspondence to M. Bonierbale.

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Communicated by C. Gebhardt.

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Mihovilovich, E., Munive, S. & Bonierbale, M. Influence of day-length and isolates of Phytophthora infestans on field resistance to late blight of potato. Theor Appl Genet 120, 1265–1278 (2010). https://doi.org/10.1007/s00122-009-1254-4

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