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Rpr1, a gene required for Rpg1-dependent resistance to stem rust in barley

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Abstract

Rpg1 is a stem rust resistance gene that has protected barley from severe losses for over 60 years in the US and Canada. It confers resistance to many, but not all, pathotypes of the stem rust fungus Puccinia graminis f. sp. tritici. A fast neutron induced deletion mutant, showing susceptibility to stem rust pathotype Pgt-MCC, was identified in barley cv. Morex, which carries Rpg1. Genetic and Rpg1 mRNA and protein expression level analyses showed that the mutation was a suppressor of Rpg1 and was designated Rpr1 (Required for P. graminis resistance). Genome-wide expression profiling, using the Affymetrix Barley1 GeneChip containing ∼22,840 probe sets, was conducted with Morex and the rpr1 mutant. Of the genes represented on the Barley1 microarray, 20 were up-regulated and 33 were down-regulated by greater than twofold in the mutant, while the Rpg1 mRNA level remained constant. Among the highly down-regulated genes (greater than fourfold), genomic PCR, RT-PCR and Southern analyses identified that three genes (Contig4901_s_at, HU03D17U_s_at, and Contig7061_s_at), were deleted in the rpr1 mutant. These three genes mapped to chromosome 4(4H) bin 5 and co-segregated with the rpr1-mediated susceptible phenotype. The loss of resistance was presumed to be due to a mutation in one or more of these genes. However, the possibility exists that there are other genes within the deletions, which are not represented on the Barley1 GeneChip. The Rpr1 gene was not required for Rpg5- and rpg4-mediated stem rust resistance, indicating that it shows specificity to the Rpg1-mediated resistance pathway.

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Acknowledgments

This is Scientific Paper No. 0301-06 from the College of Agricultural, Human, and Natural Sciences Research Center, Washington State University, Pullman, WA 99164, Project 0196. Research was supported by USDA-NRI grant No. 2004-35301-14635 and the US Barley Genome Project.

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Authors and Affiliations

  1. Department of Crop and Soil Sciences, Washington State University, Pullman, WA, 99164, USA

    L. Zhang, J. Nirmala, D. Schmierer, R. Brueggeman & A. Kleinhofs

  2. Agriculture and Agri-Food Canada, Cereal Research Centre, 195 Dafoe Road, R3T 2M9, Winnipeg, Manitoba, Canada

    T. Fetch

  3. Department of Plant Pathology, University of Minnesota, St. Paul, MN, 55108, USA

    B. Steffenson

  4. School of Molecular Biosciences, Washington State University, Pullman, WA, 99164, USA

    A. Kleinhofs

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  1. L. Zhang
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  2. T. Fetch
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  3. J. Nirmala
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  4. D. Schmierer
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  5. R. Brueggeman
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  6. B. Steffenson
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  7. A. Kleinhofs
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Correspondence to A. Kleinhofs.

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Communicated by A. Kilian

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Zhang, L., Fetch, T., Nirmala, J. et al. Rpr1, a gene required for Rpg1-dependent resistance to stem rust in barley. Theor Appl Genet 113, 847–855 (2006). https://doi.org/10.1007/s00122-006-0342-y

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  • DOI: https://doi.org/10.1007/s00122-006-0342-y

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