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Chromosomal rearrangements differentiating the ryegrass genome from the Triticeae, oat, and rice genomes using common heterologous RFLP probes

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Abstract

An restriction fragment length polymorphism (RFLP)-based genetic map of ryegrass (Lolium) was constructed for comparative mapping with other Poaceae species using heterologous anchor probes. The genetic map contained 120 RFLP markers from cDNA clones of barley (Hordeum vulgare L.), oat (Avena sativa L.), and rice (Oryza sativa L.), covering 664 cM on seven linkage groups (LGs). The genome comparisons of ryegrass relative to the Triticeae, oat, and rice extended the syntenic relationships among the species. Seven ryegrass linkage groups were represented by 10 syntenic segments of Triticeae chromosomes, 12 syntenic segments of oat chromosomes, or 16 syntenic segments of rice chromosomes, suggesting that the ryegrass genome has a high degree of genome conservation relative to the Triticeae, oat, and rice. Furthermore, we found ten large-scale chromosomal rearrangements that characterize the ryegrass genome. In detail, a chromosomal rearrangement was observed on ryegrass LG4 relative to the Triticeae, four rearrangements on ryegrass LGs2, 4, 5, and 6 relative to oat, and five rearrangements on ryegrass LGs1, 2, 4, 5, and 7 relative to rice. Of these, seven chromosomal rearrangements are reported for the first time in this study. The extended comparative relationships reported in this study facilitate the transfer of genetic knowledge from well-studied major cereal crops to ryegrass.

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Acknowledgements

We thank Dr. S. McCouch of Cornell University, Ithaca, N.Y. and the USDA probes depository, Albany, Calif. for kindly providing the heterologous RFLP probes. We also thank Dr. Shawn Kaeppler, Dr. Rebecca Abler, and Dr. Zahi Atallah for their valuable comments and critical review and Lori Evans for handling plant materials. The Wisconsin Sod Producers Association and the Wisconsin Turfgrass Association provided partial funding.

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Authors and Affiliations

  1. Department of Plant Pathology, University of Wisconsin-Madison, 1630 Linden Dr. Madison, WI, 53706, USA

    S. Sim, T. Chang, J. Curley & G. Jung

  2. National Arboretum, 3501 New York Ave. N.E., Washington, DC, 20002

    S. E. Warnke

  3. USDA-ARS National Forage Seed Production Research Center, 3450 SW Campus Way, Corvallis, OR, 97331, USA

    R. E. Barker

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  1. S. Sim
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  2. T. Chang
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  3. J. Curley
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  4. S. E. Warnke
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  5. R. E. Barker
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  6. G. Jung
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Corresponding author

Correspondence to G. Jung.

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Communicated by J.S. Heslop-Harrison

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Sim, S., Chang, T., Curley, J. et al. Chromosomal rearrangements differentiating the ryegrass genome from the Triticeae, oat, and rice genomes using common heterologous RFLP probes. Theor Appl Genet 110, 1011–1019 (2005). https://doi.org/10.1007/s00122-004-1916-1

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  • DOI: https://doi.org/10.1007/s00122-004-1916-1

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