Skip to main content
SpringerLink
Log in

Green tea polyphenols avert chronic inflammation-induced myocardial fibrosis of female rats

  • Original Research Paper
  • Published:
Inflammation Research Aims and scope Submit manuscript

Abstract

Objective

Green tea proposes anti-inflammatory properties which may attenuate chronic inflammation-induced fibrosis of vessels. This study evaluated whether green tea polyphenols (GTP) can avert fibrosis or vascular disruption along with mechanisms in rats with chronic inflammation.

Treatments

Forty 3-month-old female rats were assigned to a 2 (placebo vs. lipopolysaccharide, administration) × 2 (no GTP vs. 0.5% GTP in drinking water) factorial design for 12 weeks.

Methods

Masson’s trichrome staining evaluated myocardial fibrosis in coronary vessels and surrounding myocardium. Whole blood specimens were counted for differentials. Spleen tumor necrosis factor-α (TNF-α) mRNA expression was determined by real-time RT–PCR. Data were analyzed by two-way analysis of variance (ANOVA) followed by mean separation procedures.

Results

After 12 weeks, lipopolysaccharide administration induced myocardial fibrosis in vessels and surrounding myocardium, spleen TNF-α mRNA expression, and leukocytes, while GTP supplementation in drinking water significantly averted such observation.

Conclusions

GTP attenuates myocardial fibrosis through a suppression of chronic inflammation and innate immune responses.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Pedersen BK. The anti-inflammatory effect of exercise: its role in diabetes and cardiovascular disease control. Essays Biochem. 2006;42:105–17.

    Article  PubMed  CAS  Google Scholar 

  2. Laurat E, Poirier B, Tupin E, Caligiuri G, Hansson GK, Bariéty J, Nicoletti. In vivo downregulation of T helper cell 1 immune responses reduces atherogenesis in apolipoprotein E-knockout mice. Circulation. 2001;104:197–202.

  3. Regensteiner JG, Hiatt WR. Current medical therapies for patients with peripheral arterial disease: a critical review. Am J Med. 2002;112:49–57.

    Article  PubMed  Google Scholar 

  4. Tang J, Raines EW. Are suppressors of cytokine signaling proteins recently identified in atherosclerosis possible therapeutic targets? Trends Cardiovasc Med. 2005;15:243–9.

    Article  PubMed  CAS  Google Scholar 

  5. Kuriyama S, Shimazu T, Ohmori K, Kikuchi N, Nakaya N, Nishino Y, Tsubono Y, Tsuji I. Green tea consumption and mortality due to cardiovascular disease, cancer, and all causes in Japan. JAMA. 2006;296(10):1255–65.

    Article  PubMed  CAS  Google Scholar 

  6. Stangl V, Dreger H, Stangl K, Lorenz M. Molecular targets of tea polyphenols in the cardiovascular system. Cardiovasc Res. 2007;73(2):348–58.

    Article  PubMed  CAS  Google Scholar 

  7. Sueoka N, Suganuma M, Sueoka E, Okabe S, Matsuyama S, Imai K, Nakachi K, Fujiki H. A new function of green tea: prevention of lifestyle-related diseases. Ann N Y Acad Sci. 2001;928:274–80. Review.

    Google Scholar 

  8. Moore RJ, Jackson KG, Minihane AM. Green tea (Camellia sinensis) catechins and vascular function. Br J Nutr. 2009;102(120):1790–802.

    Article  PubMed  CAS  Google Scholar 

  9. Mineharu Y, Koizumi A, Wada Y, Iso H, Watanabe Y, Date C, Yamamoto A, Kikuchi S, Inaba Y, Toyoshima H, Kondo T, Tamakoshi A, and the JACC study group. Coffee, green tea, black tea and oolong tea consumption and risk of mortality from cardiovascular disease in Japanese men and women. J Epidemiol Community Health 2010. (Epub ahead of print).

  10. Suganuma M, Sueoka E, Sueoka N, Okabe S, Fujiki H. Mechanisms of cancer prevention by tea polyphenols based on inhibition of TNF-alpha expression. Biofactors. 2000;13(1–4):67–72.

    Article  PubMed  CAS  Google Scholar 

  11. Shen CL, Yeh JK, Samathanam C, Cao JJ, Stoecker BJ, Dagda RY, Chyu MC, Dunn DM, Wang JS. Green tea polyphenols attenuate deterioration of bone microarchitecture in female rats with systemic chronic inflammation. Osteoporos Int 2010. (Epub ahead of print).

  12. Wang JS, Luo H, Wang P, Tang L, Yu J, Huang T, Cox S, Gao W. Validation of green tea polyphenol biomarkers in a phase II human intervention trial. Food Chem Toxicol. 2008;46(1):232–40.

    Article  PubMed  CAS  Google Scholar 

  13. Shen CL, Yeh JK, Cao JJ, Tatum OL, Dagda RY, Wang JS. Green tea polyphenols mitigate bone loss of female rats in a chronic inflammation-induced bone loss model. J Nutr Biochem. 2010;21(10):968–74.

    Article  PubMed  CAS  Google Scholar 

  14. Shen CL, Wang P, Guerrieri J, Yeh JK, Wang JS. Protective effect of green tea polyphenols on bone loss in middle-aged female rats. Osteoporos Int. 2008;19(7):979–90.

    Article  PubMed  CAS  Google Scholar 

  15. Smith BJ, Lerner MR, Bu SY, Lucas EA, Hanas JS, Lightfoot SA, Postier RG, Bronze MS, Brackett DJ. Systemic bone loss and induction of coronary vessel disease in a rat model of chronic inflammation. Bone. 2006;38(3):378–86.

    Article  PubMed  CAS  Google Scholar 

  16. Hori M, Nishida K. Oxidative stress and left ventricular remodelling after myocardial infarction. Cardiovasc Res 2009;81(3):457–64. (Review).

    Google Scholar 

  17. Li R, Huang YG, Fang D, Le WD. (−)-Epigallocatechin gallate inhibits lipopolysaccharide-induced microglial activation and protects against inflammation-mediated dopaminergic neuronal injury. J Neurosci Res. 2004;78(5):723–31.

    Article  PubMed  CAS  Google Scholar 

  18. He P, Noda Y, Sugiyama K. Green tea suppresses lipopolysaccharide-induced liver injury in d-galactosamine-sensitized rats. J Nutr. 2001;131(5):1560–7.

    PubMed  CAS  Google Scholar 

  19. Hong Byun E, Fujimura Y, Yamada K, Tachibana H. TLR4 signaling inhibitory pathway induced by green tea polyphenol epigallocatechin-3-gallate through 67-kDa laminin receptor. J Immunol. 2010;185(1):33–45.

    Article  PubMed  CAS  Google Scholar 

  20. Koeberle A, Bauer J, Verhoff M, Hoffmann M, Northoff H. Werz O. Green tea epigallocatechin-3-gallate inhibits microsomal prostaglandin E(2) synthase-1. Biochem Biophys Res Commun. 2009;388(2):350–4.

    Article  PubMed  CAS  Google Scholar 

  21. Smith BJ, Lightfoot SA, Lerner MR, Denson KD, Morgan DL, Hanas JS, Bronze MS, Postier RG, Brackett DJ. Induction of cardiovascular pathology in a novel model of low-grade chronic inflammation. Cardiovasc Pathol. 2009;18(1):1–10.

    Article  PubMed  CAS  Google Scholar 

  22. Ramesh E, Geraldine P, Thomas PA. Regulatory effect of epigallocatechin gallate on the expression of C-reactive protein and other inflammation markers in an experimental model of atherosclerosis. Chem Biol Interact. 2010;183(1):125–32.

    Article  PubMed  CAS  Google Scholar 

  23. Kim IB, Kim DY, Lee SJ, Sun MJ, Lee MS, Li H, Cho JJ, Park CS. Inhibition of IL-8 production by green tea polyphenols in human nasal fibroblasts and A549 epithelial cells. Bio Pharm Bull. 2006;29(6):1120–5.

    Article  CAS  Google Scholar 

  24. Dona M, Dell’Aica I, Calabrese F, Benelli R, Morini M, Albini A, Garbisa S. Neutrophil restraint by green tea: inhibition of inflammation associated angiogenesis and pulmonary fibrosis. J Immunol. 2003;170(8):4335–41.

    PubMed  CAS  Google Scholar 

  25. Hofbauer R, Frass M, Gmeiner B, Handler S, Speiser W, Kapiotis S. The green tea extract epigallocatechin gallate is able to reduce neutrophil transmigration through monolayers of endothelial cells. Wien Klin Wochenschr. 1999;111(7):278–82.

    PubMed  CAS  Google Scholar 

  26. Ludwig A, Lorenz M, Grimbo N, Steinle F, Meiners S, Bartsch C, Stangl K, Baumann G, Stangl V. The tea flavonoid epigallocatechin-3-gallate reduces cytokine-induced VCAM-1 expression and monocyte adhesion to endothelial cells. Biochem Biophys Res Commun. 2004;316(3):659–65.

    Article  PubMed  CAS  Google Scholar 

  27. Chen HI, Hsieh SY, Yang FL, Hsu YH, Lin CC. Exercise training attenuates septic responses in conscious rats. Med Sci Sports Exerc. 2007;39(3):435–42.

    Article  PubMed  CAS  Google Scholar 

  28. Liese AM, Siddiqi MQ, Siegel JH, Denny T, Spolarics Z. Augmented TNF-alpha and IL-10 production by primed human monocytes following interaction with oxidatively modified autologous erythrocytes. J Leukoc Biol. 2001;70(2):289–96.

    PubMed  CAS  Google Scholar 

  29. Starzyk D, Korbut R, Grygelwski RJ. The role of nitric oxide in regulation of deformability of red blood cells in acute phase of endotoxaemia in rats. J Physiol Pharmacol. 1997;48(4):731–5.

    PubMed  CAS  Google Scholar 

  30. Cheng HC, Chan CM, Tsay HS, Liang HJ, Liang YC, Liu DZ. Improving effects of epigallocatechin-3-gallate on hemorheological abnormalities of aging guinea pigs. Circ J. 2007;71(4):597–603.

    Article  PubMed  CAS  Google Scholar 

  31. Droke EA, Hager KA, Lerner MR, Lightfoot SA, Stoecker BJ, Brackett DJ, Smith BJ. Soy isoflavones avert chronic inflammation-induced bone loss and vascular disease. J Inflamm (Lond). 2007;4:17.

    Article  Google Scholar 

Download references

Acknowledgments

This work was supported by the Laura W. Bush Institute for Women’s Health (CLS). We thank Raul Y. Dagda for the technical assistance in assessing mRNA expression of TNF-α.

Conflict of interest

None.

Author information

Authors and Affiliations

  1. Department of Pathology, Texas Tech University Health Sciences Center, BB 198, 3601 4th street, Lubbock, TX, 79430-9097, USA

    Chwan-Li Shen, Christina Samathanam, Suzanne Graham & Dale M. Dunn

  2. Laura W. Bush Institute for Women’s Health, Texas Tech University Health Sciences Center, Lubbock, TX, USA

    Chwan-Li Shen

  3. Molecular Pathology Program, Texas Tech University Health Sciences Center, Lubbock, TX, USA

    Owatha L. Tatum & Christine Tubb

  4. USDA ARS Grand Forks Human Nutrition Research Center, Grand Forks, ND, USA

    Jay J. Cao

  5. Department of Environmental Health Science, University of Georgia, Athens, GA, USA

    Jia-Sheng Wang

Authors
  1. Chwan-Li Shen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Christina Samathanam
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Owatha L. Tatum
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Suzanne Graham
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Christine Tubb
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Jay J. Cao
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Dale M. Dunn
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Jia-Sheng Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Chwan-Li Shen.

Additional information

Responsible Editor: Graham Wallace.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Shen, CL., Samathanam, C., Tatum, O.L. et al. Green tea polyphenols avert chronic inflammation-induced myocardial fibrosis of female rats. Inflamm. Res. 60, 665–672 (2011). https://doi.org/10.1007/s00011-011-0320-y

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00011-011-0320-y

Keywords

Search

Navigation