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Bioactive lipids and antioxidant properties of wild Egyptian Pulicaria incise, Diplotaxis harra, and Avicennia marina

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Abstract

Fatty acids, sterols and tocopherols profile of Pulicaria incise, Diplotaxis harra and Avicennia marina grown wild in Egypt were investigated. Linoleic acid followed by palmitic and oleic acids were the major fatty acids in P. incise. Oleic followed by stearic were the main acids in D. harra, while palmitic followed by oleic were the major fatty acids found in A. marina. Sterol markers of P. incise were campesterol followed by stigmasterol and β-sitosterol. In D. harra β-sitosterol followed by stigmasterol and ∆5-avensterol were the major sterols, while campesterol followed by β-sitosterol and ∆7-avensterol were main sterols of A. marina. Tocopherols were also estimated in high levels, wherein δ-tocopherol was the main tocopherol in all plants. Antioxidant properties of methanolic extracts of the plants were compared with methanolic solution of butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT) and ascorbic acid during thiocyanate assay. Moreover, methanolic extracts of the whole plants exhibited strong antiradical potential against DPPH radicals. Information provided by the present study is of importance for further investigations of P. incise, D. harra and A. marina and utilization of these plants as a raw material of bioactive compounds.

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Abbreviations

BHA:

Butylated hydroxyanisole

BHT:

Butylated hydroxytoluene

DPPH:

1,1-Diphenyl-2-picrylhydrazyl

FAMES:

Fatty acid methyl esters

FID:

Flame ionization detector

GS:

Gas chromatography

NP-HPCL:

Normal-phase high performance liquid chromatography

PUFA:

Polyunsaturated fatty acids

RSA:

Radical scavenging activity

ST:

Sterols

TL:

Total lipids

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Authors and Affiliations

  1. Biochemistry Department, Faculty of Agriculture, Zagazig University, 44511, Zagazig, Egypt

    M. F. Ramadan, M. M. A. Amer,  H. T. Mansour,  K. M. Wahdan &  W. A. El-Gleel

  2. Department of Industrial Biotechnology, Institute of Genetic Engineering and Biotechnology, Menufia University, Shebin Elkom, Egypt

    R. M. El-Sayed &  S. El-Sanhoty

Authors
  1. M. F. Ramadan
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  2. M. M. A. Amer
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  3. H. T. Mansour
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  4. K. M. Wahdan
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  5. R. M. El-Sayed
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  6. S. El-Sanhoty
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  7. W. A. El-Gleel
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Correspondence to M. F. Ramadan.

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Ramadan, M.F., Amer, M.M.A., Mansour, H.T. et al. Bioactive lipids and antioxidant properties of wild Egyptian Pulicaria incise, Diplotaxis harra, and Avicennia marina . J. Verbr. Lebensm. 4, 239–245 (2009). https://doi.org/10.1007/s00003-009-0300-0

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