Summary
An alkalophilic bacterium producing high amounts of the cell-associated β-mannosidase and extracellular β-mannanase was isolated from soil. The isolate (AM-001) that grew well in alkaline pH media was identified as a strain of Bacillus sp. The optimal cultivation temperature for enzyme production was 31° C for β-mannosidase and 37° C for β-mannanase with the optimum production medium composed of 1% konjac powder, 0.2% yeast extract, 2% Polypepton, 0.1% K2HPO4, 0.02% MgSO4 · 7H2O and 0.5% Na2CO3. Optimum pH and temperature for β-mannosidase were 7.0 and 55° C, and for β-mannanase were 9.0 and 65° C.
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Akino, T., Nakamura, N. & Horikoshi, K. Production of β-mannosidase and β-mannanase by an alkalophilic Bacillus sp.. Appl Microbiol Biotechnol 26, 323–327 (1987). https://doi.org/10.1007/BF00256662
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DOI: https://doi.org/10.1007/BF00256662