Summary
Microbial amperometric sensors for biochemical oxygen demand (BOD) determination using Bacillus subtilis or Trichosporon cutaneum cells immobilized in polyvinylalcohol have been developed. These sensors allow BOD measurements with very short response times (15–30s), a level of precision of ±5% and an operation stability of 30 days. A linear range was obtained for a B. subtilis-based sensor up to 20 mg/l BOD and for a T. cutaneum-based sensor up to 100 mg/l BOD using a glucose/glutamic acid standard.
References
APHA (1976) Standard methods for examination of water and waste water. American Public Health Association Inc., New York
harita K, Otani Y, Hikuma M, Yasuda T (1985) BOD quick estimating system utilizing microbial electrode. Instrumental Control. Water, Waste Water Treatment Transport System. Proc IAWPRC Workshop 4:529–532
Hikuma M, Suzuki H, Yasuda T, Karube I, Suzuki S (1979) Amperometric estimation of BOD by using living immobilized yeast. Eur J Appl Microbiol 8:289–297
JIS (1974) Japanese Industrial Standard Committee, Testing methods for industrial waste water, JIS K 0102 Tokyo, p 33
Karube I, Matsunaga T, Mitsuda S, Suzuki S (1977) Microbial electrode BOD sensor. Biotechnol Bioeng 19:1535–1547
Kawabata N, Nakamura N (1986) New BOD sensor utilizing a functional polymer which captures microorganisms alive. Abstracts of the International Symposium on New Sensors and Methods for Environmental characterization, Kyoto, Japan
Kulys J, Kadziauskiené K (1980) Yeast BOD sensor. Biotechnol Bioeng 22:221–226
Lowden G (1981), Tests for assessing the oxygen demand of effluents. Water Res Top 1:142–151
OECD (1981) Organization for Economic Cooperation and Development. Activated sludge, respiration inhibition test. OECD Guideline for Testing of Chemicals No. 209
Riedel K, Liebs P, Renneberg R (1985) An electrochemical method for determination of cell respiration. J Basic Microbiol 25:51–56
Strand SE, Carlson DA (1984) Rapid BOD measurement for municipal waste water samples using a biofilm electrode. J Water Pollut Control Fed 56:464–467
Zhang X, Wang Z, Jian H (1986) Microbial sensor for the BOD estimation (in Chinese) Huanjing Kexue Xuebao 6:184–192; cited in Chem Abstr, 105:158286 (1986)
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Riedel, K., Renneberg, R., Kühn, M. et al. A fast estimation of biochemical oxygen demand using microbial sensors. Appl Microbiol Biotechnol 28, 316–318 (1988). https://doi.org/10.1007/BF00250463
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DOI: https://doi.org/10.1007/BF00250463