Abstract
Callus cultures were established from seedling explants of Pergularia daemia (Forsk) Chiov on Murashige and Skoog (MS) medium supplemented with different concentrations of auxins. Optimal callus developed from leaf explants on MS medium supplemented with 2,4-D (2 mg l−1) + 2iP (0.1 mg l−1), was used for morphogenesis. Adventitious shoots were regenerated (70%) from the calli on MS medium supplemented with NAA (0.1 mg l−1)+ BAP (2 mg l−1). Individual shoots were rooted on half strength MS medium supplemented with 0.1 mg l−1 IBA. Plantlets with well developed roots were successfully transferred to soil and 50% of the transferred plants survived.
Abbreviations
- 2,4-D:
-
2,4-Dichlorophenoxy acetic acid
- 2iP:
-
2-Isopentyl adenine
- BAP:
-
Benzyle-6-aminopurine
- MS:
-
Murshige and Skoog
- KN:
-
Kinetin
- IAA:
-
Indole-3-acetic acid
- IBA:
-
Indole-3-butyric acid
- NAA:
-
Naphthalene-3-acetic acid
- TDZ:
-
Thidiazuron
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Kiranmai, C., Aruna, V., Karuppusamy, S. et al. Callus Culture and Plant Regeneration from Seedling Explants of Pergularia daemia (Forsk) Chiov. J. Plant Biochem. Biotechnol. 17, 99–101 (2008). https://doi.org/10.1007/BF03263269
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DOI: https://doi.org/10.1007/BF03263269