Abstract
Micropropagation protocol for an elite selection of Aloe vera syn A. barbadensis through enhanced axillary branching was standardized. Murashige and Skoog medium containing 1 mg l−1 BA and 0.2 mg l-1 IBA gave highest multiplication. Citric acid at 10mg l-1 and liquid medium improved the shoot multiplication. Hundred per cent microshoots produced rooted plantlets within 15 days of culture on hormone-free agar medium. Liquid medium during rooting stage decreased the number of shoots showing rooting response. The plants were successfully transferred in the soil and were morphologically similar to mother plants.
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Abbreviations
- BA:
-
benzyladenine
- Kn:
-
kinetin
- IBA:
-
3-indole butyric acid
- MS:
-
Murashige & Skoog
References
Anonymous, The wealth of India- Raw materials, CSI R New Delhi, 1A (1976) p191.
Bhattacharya AK & Hansda R, Indian Forester, 129 (2003) 93.
Meyer HJ & Staden JV, Plant Cell Tiss Org Cult, 26 (1991) 167.
Sanchez IC, Natali L & Cavallini A, Plant Sci, 55 (1988) 53.
Natali L, Sanchez IC & Cavallini A, Plant Cell Tiss Org Cult, 20 (1990) 71.
Meyer HJ & Staden JV, Plant Cell Tiss Org Cult, 26 (1991) 167.
Roy SC & Sarkar A, Scientia Hortic, 47 (1991) 107.
Richwine AM, Tipton JL & Thompson A, Hort Sci, 30 (1995) 1443.
Abrie A & Staden JV, Plant Growth Regulation, 33 (2001) 19.
Chaudhuri S & Mukundan U, Phytomorphology, 51 (2001) 155.
Murashige T & Skoog F, Physiol Plant, 115 (1962) 493.
Barna KS & Wakhlu AK, In Vitro Cell Dev Bioi-Plant, 33 (1995) 44.
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Aggarwal, D., Barna, K.S. Tissue Culture Propagation of Elite Plant of Aloe vera Linn. J. Plant Biochem. Biotechnol. 13, 77–79 (2004). https://doi.org/10.1007/BF03263197
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DOI: https://doi.org/10.1007/BF03263197