, Volume 40, Issue 3, pp 213-218,
Open Access This content is freely available online to anyone, anywhere at any time.

Protein binding to gold colloids

Abstract

Gold nanoparticles (size from 10 to 50 nm) were synthesized by three different methods and were characterized by UV-Vis spectroscopy and transmission electron microscopy (TEM). The shape and size distribution of gold nanoparticles were determined. The interaction of major storage protein extracted and purified from aleurone cells of barley with the gold colloids has been examined using absorption UV-Vis spectroscopy and TEM observations. These investigations evidence the binding of aleurone protein to gold nanoparticles. This binding might occur by electrostatic forces between the negatively charged citrate capped gold particles and the positively charged groups of the protein (e.g., arginine and lysine residues).

Dr. Ossi Horovitz is Professor of Physical Chemistry at the Babes-Bolyai University of Cluj-Napoca (Romania). His area of research includes computer chemistry (quantum chemistry, chemometry), macromolecules, physical chemistry of surfaces, nanosciences (colloidal gold).
Dr. Gheorghe Tomoaia, M.D., is Associate Professor (Reader) in Orthopedic Surgery and Traumatology at Iuliu Hatieganu University of Medicine and Pharmacy, in Cluj-Napoca, and the Head of the Orthopedic Surgery Section at Clinic Hospital for Adults in Cluj-Napoca, Romania. His research is centered on orthopedic surgery and cutting edge topics of basic science related to nanostructured materials, including nanoparticles of gold with applications in nanomedicine.
Dr. Aurora Mocanu is Associate Professor (Reader) and Scientific Researcher in the Department of Physical Chemistry at the Babes-Bolyai University of Cluj-Napoca (Romania). Her research interest is within the physical chemistry and biophysics of colloids and interfaces, and investigations by Langmuir-Blodgett technique and atomic force microscopy of nanostructured materials, including nanoparticles of gold.
Dr. Traianos Yupsanis is Associate Professor of Biochemistry in the Department of Chemistry and Director of the Biochemistry Laboratory, at the Aristotle University of Thessaloniki in Greece. His research interest is directed to enzymes (nucleases, protein kinases, NDPkinases, peroxidases), plant proteins and gold nanoparticles, with applications in biotechnology and nanoscience.
Dr. Maria Tomoaia-Cotisel is Professor of Physical Chemistry, Thermodynamics and Biophysics, and Director of the Physical Chemistry Center, at the Babes-Bolyai University of Cluj-Napoca (Romania). Her research interests are focused on wide physical chemistry topics, thermodynamics, molecular structure, biophysics and kinetics, as well as on colloid and surface chemistry, including biomembrane models, gold colloids and thin films, with applications in biotechnology and nanosciences.