Abstract
To understand the unusual features of the genes and genomes fromGonyaulax polyedra, we isolated the promoter portions of the luciferin binding protein (LBP) gene, using IPCR methods, and characterized their sequences. Five LBP genomic clones were classified into a group of genes from the LBPα family, based on the sequence homology of the coding portion of the LBP gene. They were subdivided into two groups. Southern analysis implied that the promoter region is conserved well in most LBP genes. The comparison of the promoter regions from the LBP and luciferase genes showed that, although some portions of their sequences were well conserved, these two genes did not share common features of promoter region, as is normally found in eukaryotes or prokaryotes.
Similar content being viewed by others
Literature Cited
Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403–410
Dodge JD (1965) Chromosome structure in the dinoflagellates and the problem of the mesokaryotic cell. Excerpta Med Int Congr Ser 91: 339–341
Guillard RRL, Ryther JH (1962) Studies of marine planktonic diatoms I.Cyclosella mana Hustedt andDetonula confervacea (Cleve) Gran. Canad. J Microbiol 8: 229–239
Hori H, Osawa S (1979) Evolutionary change in 5S RNA secondary structure and a phylogenic tree of 54 5S RNA species. Proc Natl Acad Sci USA 76: 381–385
Le QH, Markovic R, Hastings JW, Jovine RV, Morse D (1997) Structure and organization of the peridinin-chlo-rophyll a-binding protein gene inConyaulax polyedra. Mol Gen Genet 255: 595–604
Lee DH, Mittag M, Sczekan S, Morse D, Hastings JW (1993) Molecular cloning and genomic organization of a gene for Luciferin-binding protein from the DinoflagellateGonyaulax polyedra. J Biol Chem 268: 8842–8850
Li L, Hastings JW (1998) The structure and organization of the luciferase gene in the photosynthetic dinoflagellateGonyaulax polyedra. Plant Mol Biol 36: 275–84
Liptay S, Schmid RM, Nabel EG, Nabel GJ (1994) Transcriptional regulation of NF-kappa B2: evidence for kappa B-mediated positive and negative autoregulation. Mol Cell Biol 14: 7695–703
Machabee S, Wall L, Morse D (1994) Expression and genomic organization of a dinoflagellate gene family. Plant Mol Biol 25: 23–31
Morse D, Frits L, Hastings JW (1989a) Role of a luciferin-binding protein in the circadian bioluminescent reaction ofGonyaulax polyedra. J Biol Chem 264: 11822–11826
Morse D, Milos PM, Roux E, Hastings JW (1989b) Circadian regulation of bioluminescence inGonyaulax involve translational control. Proc Natl Acad Sci USA 86: 172–176
Rogers SO, Bendich AJ (1988) Extraction of DNA from plant tissues,In SB Gelvin, RF Schilperoort, DS Verma, eds, Plant Molecular Biology Manual, Vol I. Kluwer Academic Publisher, Belgium, A6: 1–10
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning, A Laboratory Manual, 2nd ed, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acids Res 22: 4673–4680
Vigne R, Jordan BR (1977) Partial enzyme digestion studies onEscherichia coli, Pseudomonas, Chlorella, Drosophila, HeLa and yeast 5S RNAs support a general class of 5S RNA models. J Mol Evol 10: 77–86
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Kim, S., Lee, HG. & Lee, DH. Isolation and sequence analysis of promoter DNA fragments of the Luciferin-binding protein gene inGonyaulax polyedra . J. Plant Biol. 43, 88–93 (2000). https://doi.org/10.1007/BF03030500
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF03030500