Skip to main content
SpringerLink
Log in

Detection of HCV RNA using reverse transcription and nested polymerase chain reaction in chronic non-A, non-B liver diseases in Korea

  • Serology and Epidemiology
  • HCV
  • Published:
Gastroenterologia Japonica Aims and scope Submit manuscript

Summary

To investigate the interrelationship between the anti-HCV prevalence and the HCV RNA positivity in chronic non-A, non-B (NANB) liver diseases in Korea (n=137), we performed RT-nested PCR using 4 primers in the 5′-noncoding region of HCV genome. The overall HCV RNA positive rates were 62.8%; 87.7% (57/65) of anti-HCV positive cases and 40.3% (29/72) of anti-HCV negative cases. Four patterns according to the combination of anti-HCV and HCV RNA assays could be observed; 41.6% in [anti-HCV+/ HCV RNA+], 5.8% in [anti-HCV+/HCV RNA], 21.2% in [anti-HCV+/HCV RNA], and 31.4% in [anti-HCV+/HCV RNA]. Our results suggested that HCV may be still related to chronic NANB liver diseases negative for anti-HCV, and other unknown agents, so-called non-B non-C, might be accounted for patients negative for both, although a low concentration of HCV RNA, or the presence of HCV RNA or HBV DNA in liver tissue should be excluded.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Alter HJ. The hepatitis C virus and its relationship to the clinical spectrum of NANB hepatitis. J Gastroenterol Hepatol 1990;Suppl 1:78–94.

    Google Scholar 

  2. Kuo G, Choo Q-L, Alter HJ, et al. An assay for circulating anti-bodies to a major etiologic virus of human non-A, non-B hepatitis. Science 1989;244: 362–364.

    Article  PubMed  CAS  Google Scholar 

  3. Weiner AJ, Kuo G, Bradley DW, et al. Detection of hepatitis C viral sequences in non-A, non-B hepatitis. Lancet 1990;335: 1–3.

    Article  PubMed  CAS  Google Scholar 

  4. Choo Q-L, Kuo G, Weiner AJ, et al. Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome. Science 1989;244: 359–362.

    Article  PubMed  CAS  Google Scholar 

  5. Garson JA, Tedder RS, Briggs M, et al. Detection of hepatitis C viral sequences in blood donations by “nested” polymerase chaing reaction and prediction of infectivity. Lancet 1990;335: 1419–1422.

    Article  PubMed  CAS  Google Scholar 

  6. McHutchison JG, Person JL, Govindarajan G, et al. Improved detection of hepatitis C virus antibodies in high-risk populations. Hepatology 1992;15: 19–25.

    Article  PubMed  CAS  Google Scholar 

  7. Okamoto H, Tsuda F, Machida A, et al. Antibodies against synthetic oligopeptides deduced from the putative core gene for the diagnosis of hepatitis C virus infection. Hepatology 1992;15: 180–186.

    Article  PubMed  CAS  Google Scholar 

  8. Hosein B, Fang CT, Popovsky MA, et al. Improved serodiagnosis of hepatitis C virus infection with synthetic peptide antigen from capsid protein. Proc Natl Acad Sci USA 1991;88: 3647–3651.

    Article  PubMed  CAS  Google Scholar 

  9. Katayama T, Mazda T, Kikuch S, et al. Improved serodiagnosis of non-A, non-B hepatitis by an assay detecting antibody to hepatitis C virus core antigen. Hepatology 1992;15: 391–394.

    Article  PubMed  CAS  Google Scholar 

  10. Cristiano K, Di Bisceglie AM, Hoofnagle JH, Feinstone S. Hepatitis C viral RNA in serum of patients with chronic non-A, non-B hepatitis: Detection by the polymerase chain reaction using multiple primer sets. Hepatology 1991;14: 51–55.

    Article  PubMed  CAS  Google Scholar 

  11. Kato N, Yokosuka O, Omata M, et al. Detection of hepatitis C virus ribonucleic acid in the serum by amplification with polymerase chain reaction. J Clin Invest 1990;86: 1784–1767.

    Article  Google Scholar 

  12. Hagiwara H, Hayashi N, Mita E, et al. Detection of hepatitis C virus RNA in chronic non-A, non-B liver disease. Gastroenterology 1992;102: 692–694.

    PubMed  CAS  Google Scholar 

  13. Hosoda K, Omata M, Yokosuka O, et al. Non-A, non-B chronic hepatitis is chronic hepatitis C: A sensitive assay for detection of hepatitis C virus RNA in the liver. Hepatology 1992;15: 777–781.

    Article  PubMed  CAS  Google Scholar 

  14. Lok ASF, Cheung R, Chan R, et al. Hepatitis C viremia in patients with hepatitis C infection. Hepatology 1992;15: 1007–1012.

    Article  PubMed  CAS  Google Scholar 

  15. Tibbs CJ, Johnson PJ, McFarlane IG, et al. Detection of anti-bodies to core, envelope, NS-1, NS-3 and NS-5 regions of hepatitis C virus in patients with chronic viral hepatitis. Hepatology 1991; 14: A82.

    Google Scholar 

  16. Person JL, McHutchison JG, Govindarajan S, et al. Patterns of antibody response in chronic hepatitis C infection determined with a four antigen recombinant immunoblot assay. Hepatology 1991;14: A141.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Internal Medicine, Kangnam St. Mary’s Hospital, Catholic University Medical College, Seuol, Korea

    Young Min Park, Seung Kew Yoon, Kyu Won Chung & Boo Sung Kim

Authors
  1. Young Min Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Seung Kew Yoon
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kyu Won Chung
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Boo Sung Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Park, Y.M., Yoon, S.K., Chung, K.W. et al. Detection of HCV RNA using reverse transcription and nested polymerase chain reaction in chronic non-A, non-B liver diseases in Korea. Gastroenterol Jpn 28 (Suppl 5), 12–16 (1993). https://doi.org/10.1007/BF02989197

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02989197

Keywords

Search

Navigation