The effect of storage conditions on the permeability of porcine buccal mucosa

Research Articles Articles

DOI: 10.1007/BF02976616

Cite this article as:
Lee, J., Lww, S.K. & Choi, Y.W. Arch Pharm Res (2002) 25: 546. doi:10.1007/BF02976616

Abstract

The impact of storage conditions on the permeability of porcine buccal mucosa to [3H]water and [14C]mannitol was assessed. The fresh pocine buccal tissue (fresh tissue) was obtained by utilizing pig heads within 24 hours of slaughter. The stored and frozen porcine buccal tissues (stored tissue and frozen tissue) were obtained after the storage of the tissue intact in the pig heads at 4°C or -20°C, respectively, for 24 h. The results demonstrated that the barrier properties of the porcine buccal mucosa were maintained with regard to [3H]water permeability when stored at 4°C for 24 h. However, freezing the tissue resulted in tissue damage illustrated by a significant increase in [3H]water permeability. [14C]Mannitol does not appear to be a suitable model solute to assess the ex vivo permeability of porcine buccal mucosa due to its extremely low permeability.

Key words

Buccal permeability Porcine buccal mucosa Storage Freezing Water Mannitol 

Copyright information

© The Pharmaceutical Society of Korea 2002

Authors and Affiliations

  1. 1.Department of Industrial and Physical Pharmacy, School of Pharmacy and Pharmacal SciencesPurdue UniversityWest LafayetteUSA
  2. 2.College of PharmacyChung-Ang UniversitySeoulKorea
  3. 3.Akina, Inc.Business and Technology CenterWest LafayetteUSA