Abstract
Carbohydrate analysis is important in studying structure and activity of complex polysaccharides. New analytical method was applied to get an information on the composition of polysaccharides showing antitumor activity. Monosaccharides were labeled with 7-amino-1,3-naphthalenedisulfonic acid (7-AGA) by reductive amination and separated by HPLC. Five kinds of polysaccharides fromBasidiomycetes were hydrolyzed and analyzed in combination with electrophoresis and HPLC. At the same time, alditol acetate derivatives were prepared and analyzed by gas chromatography. Two different techniques using different derivatization methods showed very similiar results. The monosaccharides fromCoriolus versicolor andLentinus eodes were mainly composed of D-glucose, while those fromGanoderma lucidium andCordyceps militaris were glucose and galactose.Phellinus linteus composed of glucose, galactose, mannose, arabinose and fucose. The HPLC method with fluorescence detector was very sensitive compared to other methods.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References Cited
Al-Hakim, A., and Linhardt, R. J., Isolation and recovery of acidic oligosaccharides from polyacrylamide gels by semi-dry electrotransfer.Electrophoresis, 11, 23–28 (1990).
Blakeney, A., Harris, P., Henry, R. and Stone, B., A simple and rapid preparation of alditol acetates for monosaccharide analysis.Carbohydr. Res., 113, 291–299 (1983).
Hase, S., Ibuki, T., and Ikenaka, T., Reexamination of the pyridylamination used for fluorescence labelling of oligosaccharides and its application to glycoprotein.J. Biochem., 95, 197–203 (1984).
Hase, S., Pyridylamination for sensitive analysis of sugar chains of glycoproteins In Imahori, K. and Skiyama, F. (Eds),Methods in Protein Sequence Analysis, Plenum Press, New York, 195–198 (1993).
Higashi, H., Ito, M., Fukaya, N., Yamagata, S. and Yamagata, T., Two-dimensional mapping by the high-performance liquid chromatography of oligosaccharides released for glycosphingolipids by the endoglycoceramidase.Anal. Biochem., 186, 355–362 (1990).
Jackson, P., The use of polyacrylamide-gel electrophoresis for the high resolution separation of reducing saccharides labeled with the fluorophore 8-aminonaphthalene-1,3,6-trisulfonic acid.Biochem. J., 270, 705–713.
Kakehi, K., Suzuki, S., Honda, S. and Lee, Y. C., Precolumn labeling of reducing carbohydrates with 1-(p-methoxy)phenyl-3-methyl-5-pyrazolone: Analysis of neutral and sialic acid-containing oligosaccharides found in glycoproteins.Anal. Biochem., 199, 256–268 (1991).
Kim, Yeong S., Lee, Kyung B. and Linhardt, R. J., Detection of chitinase activity activty fluorescence-labeled substrate on polyacrylamide gel.Kor. Biochem. J., 24, 466–471 (1991).
Kim, Yeong S., Park, Kyung S. and Kim, Jong K., Purification and characterization of β-galactosidase from green onion.Kor. Biochem. J., 26, 602–608 (1993).
Kim, Y. S., Roh, J. E. and Ann, H. S., Compositional Analysis of Polysaccharides fromSanguisorba officinalis and Its Anticoagulant Activity.Kor. J. Pharmacog., 24, 124–130 (1993).
Kim, Y. S., Liu, J., Han, X. J., Pervin, A. and Linhardt, R. J., Analysis of fluorescently labeled sugars by reversed-phase ion-pairing high performance liquid chromatography.Anal. Biochem. submitted (1994).
Lee, K. B., Al-Hakim, A., Lognathan, D. and Linhardt, R. J., A new method for sequencing linear oligosaccharides on gels using charged, fluorescent conjugates.Carbohydr. Res., 214, 155–168 (1991).
Lee, K. B., Kim, Y. S. and Linhardt, R. J., Lectin affinity electrophoresis for the separation of fluorescently labeled sugar derivatives.Anal. Biochem., 203, 206–210 (1992).
Lee, K. R., Zee, O. P., Kwak, J. H., Kim, Y. S., Park, H. K., and Youn, H. J., The polysaccharide fraction of Artemisiae species (I).Kor. J. Pharmacog., 24, 289–295 (1993).
Linhardt, R. J., Capillary electrophoresis of oligosaccharides In Lennarz, W. J., Hart, G. W. (Eds.)Methods in Enzymology, Guide to Techniques in Glycobiology, 230, Academic Press, New York, pp. 265–280 (1993).
Rademacher, T. W., Parekh, R. B. and Dwek, R. A., Glycobiology.Ann. Rev. Biochem., 57, 785–838 (1988).
Takemoto, H., Hase, S. and Ikenaka, T., Microquantitative analysis and amino sugars as fluorescent pyridylamino derivatives by high-performance liquid chromatography.Anal. Biochem., 145, 245–250 (1985).
Toshio, M. and Toshiro, Y., β-Glucan ofBasidiomycetes In Toshio, M. (Ed.)Structure and Activity of Polysaccharides, Chochang Publishing Co, Tokyo, 1–36 (1990).
Townsend, R. R., Hardy, M. R., Hindsgaul, O. and Lee, Y. C., High-performance anion-exchange chromatography of oligosaccharides using pellicular resins and pulsed amperometric detection.Anal. Biochem., 174, 459–470 (1988).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kim, Y.S., Park, K.S., Park, H.K. et al. Compositional sugar analysis of antitumor polysaccharides by high performance liquid chromatography and gas chromatography. Arch. Pharm. Res. 17, 337–342 (1994). https://doi.org/10.1007/BF02974173
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02974173