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Lipase catalyzed production of monoacylglycerols by the esterification of fish oil fatty acids with glycerol

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Abstract

In this study, we attempted the efficient production of monoacylglycerols (MAG) via the lipase-catalyzed esterification of glycerol with fatty acids obtained from sardine oil. The reaction factors that influenced MAG synthesis were the glycerol to fatty acid mole ratio, amount of enzyme, organic solvent, temperature, and the type of lipase used. Porcine pancreas lipase was selected to catalyze this reaction. The optimum conditions we determined for MAG synthesis were a glycerol to fatty acid mole ratio of 1∶6, 100 mg/mL of lipase, and 30°C in dioxane. Under these conditions, the MAG content was 68% (w/w) after 72 h of reaction. The MAGs synthesized via the lipase-catalyzed esterification of glycerol with fatty acids included monomyristin, monopamiltin, and monoolein, as identified by GCMS.

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Authors and Affiliations

  1. Faculty of Science and Biotechnology, Kangnung National University, 210-702, Gangneung, Korea

    Hee-Guk Byun

  2. Department of Chemistry, Pukyong National University, 608-737, Busan, Korea

    Tae-Kil Eom & Se-Kwon Kim

  3. Marine Bioprocess Research Center, Pukyong National University, 608-737, Busan, Korea

    Won-Kyo Jung & Se-Kwon Kim

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  1. Hee-Guk Byun
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  2. Tae-Kil Eom
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  4. Se-Kwon Kim
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Correspondence to Se-Kwon Kim.

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Byun, HG., Eom, TK., Jung, WK. et al. Lipase catalyzed production of monoacylglycerols by the esterification of fish oil fatty acids with glycerol. Biotechnol. Bioprocess Eng. 12, 491–496 (2007). https://doi.org/10.1007/BF02931345

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  • DOI: https://doi.org/10.1007/BF02931345

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