Summary
Hancornia speciosa fruit is highly desired for the juice and ice cream industry in tropical regions. A rapid reduction in germination ability ofH. speciosa seeds has been a problem for its large-scale cultivation. This paper describes anin vitro technique that may lead to an alternative propagation method forH. speciosa. Shoot apices and nodal segments from aseptically germinated young embryos were cultivatedin vitro on. Murashige and Skoog (1962) medium supplemented with growth regulators. Shoot multiplication was maintained by sequential subculture of shoot tips and nodal segments. N6-benzyladenine was the most effective cytokinin for the induction of shoot growth. N6-furfurylamino-purine, at various concentrations, yielded multiplication rates sevenfold lower than the highest multiplication rate found with N6-benzyladenine. Increased root initiation rate and root elongation was observed with the presence of γ-(indole-3) butyric acid in the half-strength Murashige and Skoog culture medium, especially at 10μM. N6-benzyladenine strongly inhibited rooting, even in the presence of γ-(indole-3) butyric acid. Thein-vitro-raised rooted plantlets were acclimatized to greenhouse environment through progressive reduction in relative humidity and later transplanted to the field.
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References
Brand, M. H. Initiating cultures ofHalesia andMalus: influence of flushing stage and benzyladenine. Plant Cell Tissue Organ Cult. 33:129–132; 1993.
Chalupa, V. European hardwood. In: Bonga, J. M.; Durzan, D.J., eds. Cell and tissue culture in forestry (forestry sciences). Vol. 3. Dordrecht, Netherlands: Martinus Njjhoff Publishers; 1987:224–246.
Chauvin, J.-E.; Salesses, G. Effect du fructose sur la micropropagation du châtainier Castaneae sp C.R. Acad. Sci. Paris, t. 306, Série III; 1984:207–212.
Correa, M.P. Dicionário das Plantas Uteis do Brasil e das Exóticas Cultivadas. Vol. 5. Rio de Janeiro, Brazil: Ministério da Agricultura; 1978:82–83.
Detrez, C. Shoot production through cutting culture and micrografting from mature tree explants inAcacia tortilis (Forsk.) Hayne subsp.raddiada (Savi) Brenan. Agroforestry Syst. 25:171–179; 1994.
Durant-Cresswell, R.; Boulay, M.; Francelet, A. Vegetative propagation ofEucalyptus. In: Bonga, J.M.; Durzan, D. J., eds. Tissue culture in forestry. The Hague: Martinus Nijhoff/Dr. W. Junk Publishers; 1982:150–181.
IRCA, Rapport annuel. Institut de Recherches sur le Caoutchouc (IRCA). Paris; 1986:1–198.
Litz, R. E. Somatic embryogenesis in tropical fruit trees. In: Henke, R. R., et al., eds. Tissue culture in forestry and agriculture: basic life sciences. Vol. 32. New York: Plenum Press; 1985:179–193.
Litz, R. E.; Jaiswal, V. S. Micropropagation of tropical and subtropical fruits. In: Debergh, P. C.; Zimmerman, R. H., eds. Micropropagation, technology and application. Dordrecht Netherlands: Kluwer Academic Publishers; 1991:247–263.
MacRae, S; van Staden, J..In vitro culture ofEucalyptus grandis: effect of gelling agents on propagation. J. Plant Physiol. 137:249–251; 1990.
Mascarenhas, A. F.; Nair, S.; Kulkarni, V. M., et al. In: Bonga, J. M. Durzan, D. J., eds. Cell and tissue culture in forestry (forestry sciences). Vol. 3. Dordrecht, Netherlands: Martinus Nijhoff Publishers; 1987:316–325.
McCown, B. H.. Birch (Betula spp.) In: Bajaj, Y. P. S., ed. Biotechnology in agriculture and forestry. Vol. 5. Trees II Berlin: Springer-Verlag; 1989:324–341.
Murashige, T; Skoog, F. A revised method for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473–497;1962.
Oliveira, L. M. Q.; Valio, I. F. M. Effects of moisture content on germination of seeds ofHancornia speciosa Gom (Apocynaceae) Ann. Bot. 69:1–5; 1992.
Paranjothy, K..Hevea tissue culture. In: Bonga, J. M.; Durzan, D. J. eds. Cell and tissue culture in forestry (forestry sciences). Vol. 3. Dordrecht, Netherlands: Martinus Nijhoff Publishers; 1987:326–337.
Parente, T. V.; Machado, J. W. B. Germinaçao de sementes de mangaba (Hancornia pubescens) provenientes de frutos colhidos com diferentes graus de maturaçao. Revta. Brasil. Frutic. 8:39–43; 1986.
Samson, J. A. Tropical fruits. London: Longman; 1986:2.
Serres, R.; Stang, E.; McCabe, D., et al. Gene transfer using electric discharge particle bombardment and recovery of transformed cranberry plants. J. Am. Soc. Hortic. Sci. 117:174–180; 1992.
Sita, G. L.; Swamy, B. V. R. Regeneration of plantlets from leaf, disc cultures of rosewood: control of leaf abscission and shoot tip necrosis. Plant Sci. 88:107–122; 1993.
Swamy, B. V. R.; Himabindu, K.; Sita, G. L.In vitro micropropagation of elite rosewood (Dalbergia latifolia Roxb). Plant Cell Rep. 11:126–131; 1992.
Thorpe, T. A.; Harry, I. S.; Kumar, P. P. Application of micropropagation to forestry. In: Debergh, P. C.; Zimmerman, R.H., eds. Micropropagation, technology and application. Dordrecht, Netherlands: Kluwer Academic Publishers; 1991:311–336.
Welander, M.; Welander, N.T.; Brackman, A.-S. Regulation ofin vitro shoot multiplication inSyringa, Alnus andMalus by different carbon sources. J. Hortic. Sci. 64:361–366; 1989.
Zaerr, J.B.; Mapes, M. O. Action of growth regulators. In: Bonga, J. M.; Durzan, D. J., eds. Tissue culture in forestry. The Hague: Martinus Nijhoff/Dr. W. Junk Publishers; 1982:231–255.
Zimmerman, R. H. Application of tissue culture propagation to woody plants. In: Henke, R. R., et al., eds. Tissue culture in forestry and agriculture: basic life sciences. Vol. 32. New York: Plenum Press; 1985:165–177.
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Pereira-Netto, A.B. In vitro propagation ofHancornia speciosa, a tropical fruit tree. In Vitro Cell.Dev.Biol.-Plant 32, 253–256 (1996). https://doi.org/10.1007/BF02822696
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DOI: https://doi.org/10.1007/BF02822696