Abstract
The genotoxicity of quinolone antibiotics (ciprofloxacin, enoxacin, nalidixic acid, norfloxacin, ofloxacin, pefloxacin) was studied on the selected mutantE. coli strain PQ37 (SOS chromotest). The genotoxicity was expressed by SOS-inducing potential (SOSIP) values. The highest SOSIP values were found with ciprofloxacin (SOSIP=1967 δIF/nmol), the lowest value was observed with nalidixic acid (SOSIP=0.3 ΔIF/nmol). Similar results were also found with the biosynthesis of nucleic acids, as indicated by incorporation of14C-adenine into TCA-insoluble fractions ofS. typhimurium cells (ciprofloxacin IC50=0.39, nalidixic acid IC50=400). DNA-damaging effects were tested in the absence of an exogenous metabolizing system.
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References
Ames B.N.: Mutagenesis and carcinogenesis endogenous and exogenous factors.Environ. Mol. Mutagen. 15, 66–77 (1989).
Ashby J., Tennant R.W.: Chemical structure, salmonella mutagenicity and extent of carcinogenicity as indicators of genotoxic carcinogenesis among 222 chemicals in rodents by U.S.NCI/NTP.Mutat. Res. 204, 17–115 (1988).
Gootz T.D., Barett J.F., Suteliffe J.A.: Inhibitory effects of quinolone antibacterial agents on eucaryotic topoisomerases and related test systems.Antimicrob. Agents Chemother. 34, 8–12 (1990).
Hussey P., Maas G., Tümmler B., Grosse F., Schomburg U.: Effect of 4-quinolones and novobiocin on calf thymus DNA polymerase I and II, and growth of mammalian lymphoblasts.Antimicrob. Agents. Chemother. 29, 1073–1078 (1986).
Christ W., Lehnert T., Ulbrich B.: Specific toxicologic aspects of the quinolones.Rev. Infect. Dis. 10, 141–146 (1988).
Majtánová L'., Hoštacká A., Majtán V.: Effect of subinhibitory concentrations of antibiotics on biological properties ofSalmonella typhimurium.Folia Microbiol. 39, 141–146 (1994).
Majtánová L'., Hoštacká A., Majtán V.: Influence of sub-MICs of beta-lactam, aminoglycoside and quinolone antibiotics on the induction of prophage and permeability factors production ofSalmonella typhimurium.Biologia 3, 211–216 (1995).
Mamber S.W., Kolek B., Brookshire K.W., Bonner D.P., Fung-Tomc J.: Activity of quinolones in the AmesSalmonella TA102 mutagenicity test and other bacterial genotoxicity assays.Antimicrob. Agents Chemother. 37, 213–217 (1993).
Mersch-Sundermann V., Hauff K.H., Braun P., Wenging L., Hof H.: DNA damage caused by antibiotic drugs: Quinolones.Interact. J. Oncol. 5, 855–859 (1994).
Power E.G.M., Phillips I.: Induction of the SOS gene (umuC) by 4-quinolone antibacterial drugs.J. Med. Microbiol. 36, 78–82 (1992).
Quillardet P., Hofnung M.: The SOS chromotest, a colorimetric bacterial assay for genotoxins: Procedures.Mutat. Res. 147, 65–78 (1985).
Shen L.L., Mitsher L.A., Sharma P.N., O'Donnell T.J., Chu C.S., Rosen A.G., Pernet A.G.: Mechanism of inhibition of DNA gyrase by quinolone antibacterials. A cooperative drug-DNA-binding model.Biochemistry 28, 2886–2894 (1989).
Staples S.J., Asher S.E., Gianella R.A.: Purification and characterization of heat-stable enterotoxin produced by a strain ofEscherichia coli pathogenic for man.J. Biol. Chem. 225, 4716–4721 (1980).
Taketo A., Watanabe H.: Effect of nalidixic acid on the growth of bacterial viruses.J. Biochem. 61, 520–522 (1967).
Walker G.C.: Mutagenesis and inducible responses to deoxyribonucleic acid damage inEscherichia coli.Microbiol. Rev. 48, 60–93 (1984).
Wolfson J.S., Hooper D.C.: Fluoroquinolone antimicrobial agents.Clin. Microb. Rev. 2, 378–424 (1989).
Yoshida H., Nakamura M., Bogaki M., Ito H., Kojima T., Hattori H., Nakamura S.: Mechanism of action of quinolones againstEscherichia coli DNA gyrase.Antimicrob. Agents Chemother. 37, 839–845 (1993).
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Majtánová, L., Majtán, V. Quinolone effects in the SOS chromotest and in the synthesis of biomacromolecules. Folia Microbiol 41, 233–236 (1996). https://doi.org/10.1007/BF02814622
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DOI: https://doi.org/10.1007/BF02814622