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Evidence for the hybrid origin ofpotamogeton ×cooperi (Potamogetonaceae): Traditional morphology-based taxonomy and molecular techniques in concert

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Abstract

The identity of plants morphologically intermediate betweenPotamogeton crispus andP. perfoliatus from two recently discovered sites, one in Moravia, Czech Republic and another in Wales, United Kingdom, was investigated with molecular markers. Evidence from restriction fragment length polymorphism analysis of the nuclear internal transcribed spacer region of ribosomal DNA and of thetrnK-trnQ chloroplast DNA intergenic spacer confirmed the morphology-based determination of two putative hybrid samples asP. ×cooperi. The hybrids showed the ITS variants of both parental taxa, consistent with the expected biparental inheritance of nuclear DNA. The chloroplast DNA markers indicateP. crispus as the female parent in both hybridization events. The hybrid origin of another dubious sample was excluded by the molecular data, in accordance with previous detailed morphological examination. This plant represented an extreme, narrow-leaved form ofP. perfoliatus, imitatingP. ×cooperi in some characters. The results of the molecular analyses are discussed in relation to the morphology of the plants. They underline that somePotamogeton hybrids could indeed be identified by careful and detailed morphological examination and also that these identifications were reliable and confirmed by molecular markers. This study exemplifies that long-term taxonomic expertise usually generates very well-founded specific questions suitable for straightforward treatment by appropriate molecular methods. The process and ecological implications of hybrid formation are also discussed

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References

  • Ascherson P. &Graebner P. (1897):Synopsis der mitteleuropäischen Flora 1. Wilhelm Engelman, Leipzig.

    Google Scholar 

  • Álvarez I. &Wendel J.F. (2003): Ribosomal ITS sequences and plant phylogenetic inference.Molec. Phylogenet. Evol. 29: 417–434.

    Article  PubMed  CAS  Google Scholar 

  • Baagøe J. (1897):Potamogeton undulatusWolfgang.Bot. Tidsskr. 21: 221–236, pl. 7.

    Google Scholar 

  • Baumel A., Ainouche M.L. &Levasseur J.E. (2001): Molecular investigations in populations ofSpartina anglicaC.E. Hubbard (Poaceae) invading coastal Brittany (France).Molec. Biol. 10: 1689–1701.

    CAS  Google Scholar 

  • Birky C.W. Jr. (1995): Uniparental inheritance of mitochondrial and chloroplast genes: Mechanisms and evolution.Proc. Natl. Acad. Sci. USA 92: 11331–11338.

    Article  PubMed  CAS  Google Scholar 

  • Campbell C.S., Wojciechowski M.F., Bladwin B.G., Alice L.A. &Donoghue M.J. (1997): Persistent nuclear ribosomal DNA sequence polymorphism in theAmelanchier agamic complex.Molec. Biol. Evol. 14: 81–90.

    PubMed  CAS  Google Scholar 

  • Dandy J.E. (1975):Potamogeton L. In:Stace C.A. (ed.),Hybridization and the flora of the British Isles, Academic Press, London, pp. 444–459.

    Google Scholar 

  • Dandy J.E. (1980): 1.Potamogeton L. In:Tutin T.G., Heywood V.H., Burges N.A., Moore D.M., Valentine D.H., Walters S.M. &Webb D.A. (eds.),Flora Europaea 5: Alismataceaeto Orchidaceae (Monocotyledones), Cambridge University Press, Cambridge, pp. 7–11.

    Google Scholar 

  • Dandy J.E. &Taylor G. (1946): An account of ×Potamogeton suecicusRicht. in Yorkshire and the Tweed.Trans. & Proc. Bot. Soc. Edinburgh 34: 348–360.

    Google Scholar 

  • Dumolin-Lapegue S., Pemonge M.-H. &Petit R.J. (1997): An enlarged set of consensus primers for the study of organelle DNA in plants.Molec. Ecol. 6: 393–397.

    Article  CAS  Google Scholar 

  • Fant J.B., Preston C.D. &Barrett J.A. (2001a): Isozyme evidence for the origin ofPotamogeton ×sudermanicus as a hybrid betweenP. acutifolius andP. berchtoldii.Aquatic Bot. 71: 199–208.

    Article  CAS  Google Scholar 

  • Fant J.B., Preston C.D. &Barrett J.A. (2001b): Isozyme evidence of the parental origin and possible fertility of the hybridPotamogeton × fluitansRoth.Pl. Syst. Evol. 229: 45–57.

    Article  CAS  Google Scholar 

  • Fant J.B., Kamau E.M. &Preston C.D. (2003): Chloroplast evidence for the multiple origins of the hybridPotamogeton × sudermanicusHagstr.Aquatic Bot. 75: 351–356.

    Article  CAS  Google Scholar 

  • Fischer G. (1904): Beitrag zur Kenntnis der bayerischen Potamogetoneen IV.Mitt. Bayer. Bot. Ges. 1: 356–366 & 375–388.

    Google Scholar 

  • Fischer G. (1905): Beitrag zur Kenntnis der bayerischen Potamogetoneen V.Mitt. Bayer. Bot. Ges. 1: 471–475.

    Google Scholar 

  • Fischer G. (1907): Die bayerischen Potamogetonen und Zannichellien.Ber. Bayer. Bot. Ges. 11: 20–162.

    Google Scholar 

  • Franzke A. &Mummenhoff K. (1999): Recent hybrid speciation inCardamine (Brassicaceae) — conversion of nuclear ribosomal ITS sequences in statu nascendi.Theor. Appl. Genet. 98: 831–834.

    Article  CAS  Google Scholar 

  • Fryer A. (1890): Supposed hybridity inPotamogeton.J. Bot. 28: 173–179.

    Google Scholar 

  • Fryer A. (1891): On a new BritishPotamogeton of thenitens group.J. Bot. 29: 289–292, t. 313.

    Google Scholar 

  • Fryer A. (1897):Potamogeton undulatus,Wolf. =P. perfoliatus × crispus.Rep. Bot. Soc. Exch. Club Brit. Isles 1: 497.

    Google Scholar 

  • Galinis V. (1963):1 šeima. Pludiniai — PotamogetonaceaeEngl. In:Natkevičaitė-IvanauskienėM. (ed.),Lietuvos TSR Flora (Flora of Lithuania) 2, Lietuvos TSR Mokslu Akademija Botanikos Institutas, Vilnius, pp. 35–84 & 677.

    Google Scholar 

  • Glück H. (1936): Pteridophyten und Phanerogamen. In:Pascher A. (ed.),Süßwasserflora Mitteleuropas 15, Jena.

  • Graebner P. (1907): 4.Potamogeton (Tourn.) L. In:Engler A. (ed.),Das Pflanzenreich, Regni vegetabilis conspectus 31 (IV. 11), Berlin, pp 39–142 & 161–162.

  • Hagström J.O. (1916): Critical researches on the Potamogetons.Kungl. Svenska Vetenskapsakad. Handl. 55(5): 1–281.

    Google Scholar 

  • Hall T.A. (1999): BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT.Nucl. Acids Symp. Series 41: 95–98.

    CAS  Google Scholar 

  • Haynes R.R. (1985): A revision of the clasping-leavedPotamogeton (Potamogetonaceae).Sida 11: 173–188.

    Google Scholar 

  • Haynes R.R. &Hellquist C.B. (2000): 195.PotamogetonaceaeDumortier. In:Flora of North America Editorial Committee (eds.),Flora of North America north of Mexico 22, Oxford University Press, New York, pp. 47–74.

    Google Scholar 

  • Hejný S. (1950): Čeled’ 138.Potamogetonaceae. Rdestovité — Rudavcovité. In:Dostál. J. (ed.),Květena ČSR (Flora of the Czechoslovakia) 2, ČSBS, Praha, pp. 1722–1737.

    Google Scholar 

  • Heslop-Harrison J.W. &Clark W.A. (1941): Hybrid Potamogetons on the Isle of Benbecula.Occas. Notes Dept. Bot., King’s Coll., Newcastle upon Tyne 2: 1–4.

    Google Scholar 

  • Hettiarachchi P. &Triest L. (1991): Isozyme polymorphism in the genusPotamogeton (Potamogetonaceae).Opera Bot. Belg. 4: 87–114.

    Google Scholar 

  • Hollingsworth P.M., Gornall R.J. &Preston C.D. (1995a): Genetic variability in British populations ofPotamogeton coloratus (Potamogetonaceae).Pl. Syst. Evol. 197: 71–85.

    Article  CAS  Google Scholar 

  • Hollingsworth P.M., Preston C.D. &Gornall R.J. (1995b): Isozyme evidence for hybridization betweenPotamogeton natans andP. nodosus (Potamogetonaceae) in Britain.Bot. J. Linn. Soc. 117: 59–69.

    Article  Google Scholar 

  • Hollingsworth P.M., Preston C.D. &Gornall R.J. (1996a): Genetic variability in two hydrophilous species ofPotamogeton, P. pectinatus andP. filiformis (Potamogetonaceae).Pl. Syst. Evol. 202: 233–254.

    Article  CAS  Google Scholar 

  • Hollingsworth P.M., Preston C.D. &Gornall R.J. (1996b): Isozyme evidence for the parentage and multiple origins ofPotamogeton ×suecicus (P. pectinatus × P. filiformis, Potamogetonaceae).Pl. Syst. Evol. 202: 219–232.

    Article  CAS  Google Scholar 

  • Hollingsworth P.M., Preston C.D. &Gornall R.J. (1998): Euploid and aneuploid evolution inPotamogeton (Potamogetonaceae): a factual basis for interpretation.Aquatic Bot. 60: 337–358.

    Article  Google Scholar 

  • Iida S. &Kadono Y. (2002): Genetic diversity and origin ofPotamogeton anguillanus (Potamogetonaceae) in Lake Biwa, Japan.J. Plant Res. 115: 11–16.

    Article  PubMed  CAS  Google Scholar 

  • Kalkman L. &van Wijk R.J. (1984): On the variation on chromosome number inPotamogeton pectinatus L.Aquatic Bot. 20: 343–349.

    Article  Google Scholar 

  • Kaplan Z. (2001):Potamogeton × fluitans (P. natans × P. lucens) in the Czech Republic. I. Morphology and anatomy.Preslia 73: 333–340.

    Google Scholar 

  • Kaplan Z. (2002a): Phenotypic plasticity inPotamogeton (Potamogetonaceae).Folia Geobot. 37: 141–170.

    Article  Google Scholar 

  • Kaplan Z. (2002b): 147.PotamogetonaceaeDum. — rdestovité. In:KubátK., HroudaL., ChrtekJ. jun.,KaplanZ., KirschnerJ. &ŠtěpánekJ. (eds.),Klíč ke květeně České republiky (Key to the flora of the Czech Republic), Academia, Praha, pp. 736–743.

    Google Scholar 

  • Kaplan Z. &Štěpánek J. (2003): Genetic variation within and between populations ofPotamogeton pusillus agg.Pl. Syst. Evol. 239: 95–112.

    Article  CAS  Google Scholar 

  • Kaplan Z. &Wolff P. (2004): A morphological, anatomical and isozyme study ofPotamogeton × schreberi: confirmation of its recent occurrence in Germany and first documented record in France.Preslia 76: 141–161.

    Google Scholar 

  • Kaplan Z., Plačková I. &Štěpánek J. (2002):Potamogeton × fluitans (P. natans × P. lucens) in the Czech Republic. II. Isozyme analysis.Preslia 74: 187–195.

    Google Scholar 

  • King R.A., Gornall R.J., Preston C.D. &Croft J.M. (2001): Molecular confirmation ofPotamogeton × bottnicus (P. pectinatus × P. vaginatus, Potamogetonaceae) in Britain.Bot. J. Linn. Soc. 135: 67–70.

    Article  Google Scholar 

  • Les D.H. &Philbrick C.T. (1993): Studies of hybridisation and chromosome number variation angiosperms: evolutionary implications.Aquatic Bot. 44: 181–228.

    Article  Google Scholar 

  • Nováková H. (1982): Rozšíření vybraných širokolistých rdestů (Potamogeton L.) v ČSR (Distribution of selected broad-leaved pondweeds (Potamogeton L.) in the Czech Republic).Práce Stud., Přír. (Pardubice) 13–14: 49–71.

    Google Scholar 

  • Ogden E.C. (1943): The broad-leaved species ofPotamogeton of North America north of Mexico.Rhodora 45: 57–105, 119–163 & 171–214.

    Google Scholar 

  • Ploeg D.T.E. van der (1990): De Nederlandse breedbladige Fonteinkruiden.Wetensch. Meded. Kon. Ned. Natuurhist. Ver. 195: 1–98.

    Google Scholar 

  • Preston C.D. (1988a): Alfred Fryer and the study of the genusPotamogeton of the British Islcs.Arch. Nat. Hist. 15: 15–33.

    Article  Google Scholar 

  • Preston C.D. (1988b): ThePotamogeton taxa described by Alfred Fryer.Watsonia 17: 23–35.

    Google Scholar 

  • Preston C.D. (1995):Pondweeds of Great Britain and Ireland. Botanical Society of the British Isles, London.

    Google Scholar 

  • Preston C.D. (2001): [review] Flora of North America north of Mexico. Volume 22Magnoliophyta: Alismatidae, Arecidae, Commelinidae (in part), andZingiberaceae.Watsonia 23: 458–459.

    Google Scholar 

  • Preston C.D., Bailey J.P. &Hollingsworth P.M. (1998a): A reassessment of the hybridPotamogeton × gessnacensisG. Fisch. (P. natans × P. polygonifolius, Potamogetonaceae) in Britain.Watsonia 22: 61–68.

    Google Scholar 

  • Preston C.D. &Chater A.O. (1997):Potamogeton × cooperi(Fryer) Fryer in Wales.Bot. Soc. Brit. Isles Welsh Bull. 62: 11–12.

    Google Scholar 

  • Preston C.D. &Croft J.M. (1997):Aquatic plants in Britain and Ireland. Harley Books, Colchester.

    Google Scholar 

  • Preston C.D., Hollingsworth P.M. &Gornall R.J. (1998b):Potamogeton pectinatus L. ×P. vaginatusTurcz (P. × bottnicusHagstr.), a newly identified hybrid in the British Isles.Watsonia 22: 69–82.

    Google Scholar 

  • Preston C.D., Hollingsworth P.M. &Gornall R.J. (1999): The distribution and habitat ofPotamogeton × suecicusK. Richt. (P. filiformisPers. ×P. pectinatus L.) in the British Isles.Watsonia 22: 329–342.

    Google Scholar 

  • Raunkiaer C. (1896):De Danske blomsterplanters naturhistorie (Natural history of Danish flowering plants). I. Helobiae. Kjobenhavn.

    Google Scholar 

  • Raunkiaer C. (1903): AnatomicalPotamogeton-studies andPotamogeton fluitans.Bot. Tidssk. 25: 253–280.

    Google Scholar 

  • Štorchová H., Hrdličková R., Chrtek J. Jr.,Tetera M., Fitze D. &Fehrer J. (2000): An improved method of DNA isolation from plants collected in the field and conserved in saturated NaCl/CTAB solution.Taxon 49: 79–84.

    Article  Google Scholar 

  • Thompson J.D., Gibson T.J., Plewniak F., Jeanmougin F. &Higgins D.G. (1997): The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quailty analysis tools.Nucl. Acids Res. 25: 4876–4882.

    Article  PubMed  CAS  Google Scholar 

  • Topa E. (1966): Fam. 112.PotamogetonaceaeA.L. Juss. In:SąvulescuT. (ed.),Flora republicii socialiste Romania (Flora of Romania) 11, Acad. Reip. Soc. Rom., Bucuresti, pp. 54–88.

    Google Scholar 

  • van Wijk R.J., van Goor E.M.J. &Verkley J.A.C. (1988): Ecological studies onPotamogeton pectinatus L. II. Autecological characteristics, with emphasis on salt tolerance, intraspecific variation and isoenzyme patterns.Aquatic Bot. 32: 239–260.

    Article  Google Scholar 

  • Vargas P., McAllister H.A., Morton C., Jury S.L. &Wilkinson M.J. (1999): Polyploid speciation inHedera (Araliaceae): phylogenetic and biogeographic insights based on chromosome counts and ITS sequences.Pl. Syst. Evol. 219: 165–179.

    Article  CAS  Google Scholar 

  • Wendel J.F. (2000): Genome evolution in polyploids.Pl. Molec. Biol. 42: 225–249.

    Article  CAS  Google Scholar 

  • Wendel J.F., Schnabel A. &Seelanan T. (1995): Bidirectional interlocus concerted evolution following allopolyploid speciation in cotton (Gossypium).Proc. Natl. Acad. Sci. USA 92: 280–284.

    Article  PubMed  CAS  Google Scholar 

  • White T.J., Bruns T., Lee S. &Taylor J. (1990): Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetic. In:Innis M.A., Gelfand D.H., Sninsky J.J. &White T.J. (eds.),PCR Protocols: A guide to methods and applications, Academic Press, San Diego, pp. 313–322.

    Google Scholar 

  • Wiegleb G. (1990): The importance of stem anatomical characters for the systematics of the genusPotamogeton L.Flora 184: 197–208.

    Google Scholar 

  • Wiegleb G. &Kaplan Z. (1998): An account of the species ofPotamogeton L. (Potamogetonaceae).Folia Geobot. 33: 241–316.

    Google Scholar 

  • Wolfe Murphy S.A., Smith S.J. &Preston C.D. (1991): Irish pondweeds: 1. A recent record ofPotamogeton × cooperi (Fryer)Fryer from Co. Antrim.Irish. Nat. J. 23: 457–458.

    Google Scholar 

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  1. Institute of Botany, Academy of Sciences of the Czech Republic, CZ-252 43, Průhonice, Czech Republic

    Zdeněk Kaplan & Judith Fehrer

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  1. Zdeněk Kaplan
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Kaplan, Z., Fehrer, J. Evidence for the hybrid origin ofpotamogeton ×cooperi (Potamogetonaceae): Traditional morphology-based taxonomy and molecular techniques in concert. Folia Geobot 39, 431–453 (2004). https://doi.org/10.1007/BF02803212

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Nomenclature and hybrid formulas

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