Summary
A method based on the use of a neutral bacterial protease was employed for the preparation of isolated human hepatocytes from surgical biopsy specimens. Cells maintained in primary culture developed ultrastructural features characteristic of human hepatocytes in vivo. They retained the ability to bind insulin; binding kinetics were similar to those of other species. The hepatocytes responded to physiological concentrations of insulin by an increase in glycogen synthesis.
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Maekubo, H., Ozaki, S., Mitmaker, B. et al. Preparation of human hepatocytes for primary culture. In Vitro Cell.Dev.Biol.-Plant 18, 483–491 (1982). https://doi.org/10.1007/BF02796477
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DOI: https://doi.org/10.1007/BF02796477