Summary
The pathogenesis ofPseudomonas aeruginosa for the respiratory tract has been examined using hamster tracheal organ cultures. Tracheal rings prepared from male Syrian hamsters, strain LSH/LAK, were infected withP. aeruginosa for 4 h and processed at 4-h intervals for 24 h for examination by light- and electron microscopy. Tissue destruction was observed within 8 h after infection with 108 colony-forming units (cfu)/ml and within 12 h after infection with 104 or 106 cfu/ml. Ciliated cells that contained abnormal subcellular organelles were expelled from the epithelium. By 20 h the epithelial borders were composed primarily of nonciliated cells. Transmission- and scanning electron microscopy revealed details of the cellular destruction and attachment ofP. aeruginosa to the ciliated epithelium.Pseudomonas aeruginosa causes a rapid destruction of the epithelium of hamster trachea in cultures. Hamster tracheal organ cultures have been shown to be useful in studying the pathogenesis ofP. aeruginosa for the respiratory tract.
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This work was supported by Grants G-430B and G-431B from the Cystic Fibrosis Foundation.
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Baker, N.R., Tao, Y. A tracheal culture model of respiratory tract infection withPseudomonas Aeruginosa . In Vitro 18, 369–376 (1982). https://doi.org/10.1007/BF02796337
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DOI: https://doi.org/10.1007/BF02796337