, Volume 18, Issue 10, pp 827-834

Establishment and characterization of chicken embryo fibroblast clone LSCC-H32

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Summary

Cell line CEC-32 and clone LSCC-H32 were established from primary chicken embryo cells spontaneously but not experimentally transformed at 32° C. The lines consisted of fibroblastoid and polygonal cells and had a subtetraploid karyotype of 2N=130 to 140. The cells showed increased plating efficiency and metabolic activities as demonstrated by hexose uptake and plasminogen activator assay. The established cells produced avian lymphoid leukosis viruses of subgroups A and B. The virus released from LSCC-H32 cells induced lymphoid leukosis in inoculated chickens 18 to 22 wk post infection (PI). The cells have been carried in continuous culture for 285 passages and they appeared to grow indefinitely. They were efficiently used to propagate several animal viruses and to titrate chicken interferon.

We appreciate the advice and help of L. B. Crittenden, East Lansing, MI, and G. deBoer, Lelystad, The Netherlands, for the subgroup determination of the avian retrovirus; of H.-J. Msrschall, Hannover, Germany, for the electron microscopy; of H. Ch. Löhliger Celle, for histological examinations; of C. B. Boschek, Giessen, for staining of cytoskeletons; and of J. H. Cox for his critical reading of the manuscript.
The work was supported by Bundesministerium für Forschung und Technologie, Bonn (Grant PTB 8066).