Journal of Biosciences

, Volume 7, Issue 1, pp 39–47

Isolation, characterization and mapping of temperature-sensitive mutants of mycobacteriophage I3

  • Chanchal Sadhu
  • Mohan lal Gope
  • Krishna Sadhu
  • K. P. Gopinathan
Article

DOI: 10.1007/BF02716765

Cite this article as:
Sadhu, C., Gope, M.., Sadhu, K. et al. J Biosci (1985) 7: 39. doi:10.1007/BF02716765

Abstract

Eighteen temperature-sensitive mutants of mycobacteriophage I3 have been isolated and partially characterized. All the mutants were defective in vegetative replication. Based on temperature shift experiments with the temperature sensitive mutants, the thermosensitive phase of the phage development period has been characterized for each mutant. The genes have been mapped by recombination analysis. The early, continuous and middle genes seem to cluster on the genetic map

Keywords

Mycobacteriophage I3temperature sensitive mutantsrecombination analysisgene mapping

Abbreviations used

ts

Temperature-sensitive

moi

multiplicities of infection

pfu

plaque forming unit

NTG

N-methyl-N′-nitro-N-nitrosoguanidine

KCN

potassium cyanide

Copyright information

© Indian Academy of Sciences 1985

Authors and Affiliations

  • Chanchal Sadhu
    • 1
  • Mohan lal Gope
    • 1
  • Krishna Sadhu
    • 1
  • K. P. Gopinathan
    • 1
  1. 1.Microbiology and Cell Biology LaboratoryIndian Institute of ScienceBangaloreIndia