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Isolation, characterization and mapping of temperature-sensitive mutants of mycobacteriophage I3

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Abstract

Eighteen temperature-sensitive mutants of mycobacteriophage I3 have been isolated and partially characterized. All the mutants were defective in vegetative replication. Based on temperature shift experiments with the temperature sensitive mutants, the thermosensitive phase of the phage development period has been characterized for each mutant. The genes have been mapped by recombination analysis. The early, continuous and middle genes seem to cluster on the genetic map

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Abbreviations

ts:

Temperature-sensitive

moi:

multiplicities of infection

pfu:

plaque forming unit

NTG:

N-methyl-N′-nitro-N-nitrosoguanidine

KCN:

potassium cyanide

References

  • Adams, M. H. (1959)Bacteriophages, (New York: Interscience).

    Google Scholar 

  • Brewer, G. J. (1978)Mol. Gen. Genet.,167, 65.

    PubMed  CAS  Google Scholar 

  • Edgar, R. S. and Lielausis, I. (1964)Genetics,49, 649.

    PubMed  CAS  Google Scholar 

  • Eisen, H. A., Fuerst, C. R., Siminovitch, L, Thomas, R., Lambert, L., Pereira Da Silva, L. and Jacob, F. (1966)Virology,30, 224.

    Article  PubMed  CAS  Google Scholar 

  • Gopinathan, K. P. (1981)Curr. Sci.,50, 216.

    CAS  Google Scholar 

  • Gopinathan, K. P., Saroja, D., Gadagkar, R. and Ramakrishnan, T. (1978) inGenetics of Actinomycetales, (eds E. Freerkscen, I. Tarnok and J. H. Thumin) (New York: G. Fischer, Stuttgart), p. 237.

    Google Scholar 

  • Karnik, S. S. and Gopinathan, K. P. (1980)J. Virol.,33, 969.

    PubMed  CAS  Google Scholar 

  • Levine, M. (1961)Virology,13, 493.

    Article  PubMed  CAS  Google Scholar 

  • Lewin, B. (1977)Gene Expression. Plasmids and Phages (New York: John Wiley and Sons) Vol. 3, p. 565.

    Google Scholar 

  • Lindqvist, B. H. and Sinsheimer, R. L. (1967)J. Mol. Biol.,30, 69.

    Article  PubMed  CAS  Google Scholar 

  • Mizuguchi, Y. and Sellers, M. I. (1970) inHost-Virus Relationship in Mycobacterium, Nocardia and Actinomycetes, (eds S. E. Juhasz and G. Plummer) (Springfield: Thomas, C. C.) p. 65.

    Google Scholar 

  • Nagaraja, V. (1981)Interaction between mycobacteriophage I3and its host Mycobacterium smegmatis, Ph.D. Thesis, Indian Institute of Science, Bangalore, India.

    Google Scholar 

  • Nagaraja, V. and Gopinathan, K. P. (1980)Arch. Microbiol.,124, 249.

    Article  PubMed  CAS  Google Scholar 

  • Nagaraja, V. and Gopinathan, K. P. (1981a)FEBS Lett.,127, 57.

    Article  PubMed  CAS  Google Scholar 

  • Nagaraja, V. and Gopinathan, K. P. (1981b)Mol. Biol. Rep.,8, 11.

    Article  PubMed  CAS  Google Scholar 

  • Sadhu, C. (1984)Characterization of mycobacteriophage I3genome and its interaction with the host, Mycobacterium smegmatis, Ph.D. Thesis, Indian Institute of Science, Bangalore, India.

    Google Scholar 

  • Sunderraj, C. V. and Ramakrishnan, T. (1970)Nature (London),228, 280.

    Google Scholar 

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Authors and Affiliations

  1. Microbiology and Cell Biology Laboratory, Indian Institute of Science, 560 012, Bangalore, India

    Chanchal Sadhu, Mohan lal Gope, Krishna Sadhu & K. P. Gopinathan

Authors
  1. Chanchal Sadhu
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  2. Mohan lal Gope
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  3. Krishna Sadhu
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  4. K. P. Gopinathan
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Sadhu, C., Gope, M.l., Sadhu, K. et al. Isolation, characterization and mapping of temperature-sensitive mutants of mycobacteriophage I3. J Biosci 7, 39–47 (1985). https://doi.org/10.1007/BF02716765

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  • DOI: https://doi.org/10.1007/BF02716765

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