Article

Journal of Biosciences

, Volume 7, Issue 1, pp 39-47

Isolation, characterization and mapping of temperature-sensitive mutants of mycobacteriophage I3

  • Chanchal SadhuAffiliated withMicrobiology and Cell Biology Laboratory, Indian Institute of Science
  • , Mohan lal GopeAffiliated withMicrobiology and Cell Biology Laboratory, Indian Institute of Science
  • , Krishna SadhuAffiliated withMicrobiology and Cell Biology Laboratory, Indian Institute of Science
  • , K. P. GopinathanAffiliated withMicrobiology and Cell Biology Laboratory, Indian Institute of Science

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Abstract

Eighteen temperature-sensitive mutants of mycobacteriophage I3 have been isolated and partially characterized. All the mutants were defective in vegetative replication. Based on temperature shift experiments with the temperature sensitive mutants, the thermosensitive phase of the phage development period has been characterized for each mutant. The genes have been mapped by recombination analysis. The early, continuous and middle genes seem to cluster on the genetic map

Keywords

Mycobacteriophage I3 temperature sensitive mutants recombination analysis gene mapping