Abstract
The activation of blood neutrophil leukocytes has been proven in subjects with IgE-mediated and non-IgE-mediated asthma. This event appears to be modulated by the release of humoral factors. We submitted 12 toluene-diisocyanate (TDI) asthmatic workers to TDI provocation. During the late asthmatic reaction there was release of a serum chemoattracting factor for normal neutrophil leukocytes and activation of asthmatic neutrophil leukocytes. This appears to be the first demonstration of neutrophil chemotactic activity liberated during the late TDI reaction in humans. The results are explained by an acute inflammatory process occurring during the late asthmatic reaction induced by TDI.
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References
Agrawal KP, Mehta D, Gupta S, Chhabra SK (1986) Metabolic basis for asthma and rhinitis: an integrated approach. Lung 164:279–291
Ameille J, Brochard P, De Palmas J, Proteau J (1985) Toxicite’ des isocyanates chez l’homme. Arch Mal Prof 46:385–391
Atkins PC, Norman M, Weiner H, Zweiman B (1977) Release of neutrophil chemotactic activity during immediate hypersensitivity reactions in humans. Ann Intern Med 86:415–418
Bernstein IL (1982) Isocyanate-induced pulmonary disease: a current perspective. J Allergy Clin Immunol 70:24–31
Carroll MP, Durham SR, Walsh G, Kay AB (1985) Activation of neutrophils and monocytes after allergen- and histamine-induced bronchoconstriction. J Allergy Clin Immunol 75:290–296
Chan-Yeung M, Lam S (1986) Occupational asthma. Am Rev Respir Dis 133:686–703
Colditz IA, Movat HZ (1984) Kinetics of neutrophil accumulation in acute inflammatory lesions induced by chemotaxins and chemotaxinigens. J Immunol 133:2169–2173
Durham SR, Carroll M, Walsh GM, Kay AB (1984) Leukocyte activation in allergen-induced late-phase asthmatic reactions. N Engl J Med 311:1398–1402
Fabbri LM, Chiesura-Corona P, Dal Vecchio L, Di Giacomo GR, Zocca E, De Marzo N, Maestrelli P, Mapp CE (1985) Prednisone inhibits late asthmatic reactions and the associated increase in airway responsiveness induced by toluene-diisocyanate in sensitized subjects. Am Rev Resp Dis 132:1010–1014
Ferrante A, Thong YH (1980) Optimal conditions for simultaneous purification of mononuclear and polymorphonuclear leucocytes from human peripheral blood by the Hypaque-Ficoll method. J Immunol Methods 36:109–117
Gordon T, Sheppard D, McDonald DM, Distefano S, Scypinski L (1985) Airway hyperresponsiveness and inflammation enduced by toluene diisocyanate in guinea pigs. Am Rev Respir Dis 132:1106–1112
Governa M, Valentino M, Visonà I, Rocco M (1986) Impairment of human polymorphonuclear leukocyte chemotaxis by 2,5-hexanedione. Cell Biol Toxicol 2:33–39
Governa M, Valentino M, Visonà I (1987) In vitro impairment of human granulocyte functions by lead. Arch Toxicol 59:421–425
Hill HR, Hogan NA, Mitchell TG (1975) Evaluation of a cytocentrifuge method for measuring neutrophil granulocyte chemotaxis. J Lab Clin Med 86:703–710
Hollingsworth HM, Downing ET, Braman SS, Glassroth J, Binder R, Center DM (1984) Identification and characterization of neutrophil chemotactic activity in aspirin-induced asthma. Am Rev Respir Dis 130:373–379
Hugli TE, Morgan EL (1984) Mechanisms of leukocyte regulation by complement-derived factors. Contemp Top Immunobiol 14:109–153
Karol MH (1986) Respiratory effects of inhaled isocyanates. CRC Crit Rev Toxicol 16:349–379
Lee TH, Nagakura T, Papageorgiu N, Iikura Y, Kay AB (1983) Exercise-induced late asthmatic reactions with neutrophil chemotactic activity. N Engl J Med 308:1502–1505
Maderazo EG, Woronick CL (1978) A modified micropore filter assay of human granulocyte leukotaxis. In: Gallin I, Quie PG (eds) Leukocyte chemotaxis. Raven Press, New York, pp. 43–55
Mapp ME, Polato R, Maestrelli P, Hendrick DJ, Fabbri ML (1985) Time course of the increase in airway responsiveness associated with late asthmatic reactions to toluene diisocyanate in sensitized subjects. J Allergy Clin Immunol 75:568–572
Marcali K (1957) Microdetermination of toluene diisocyanate in the atmosphere. Anal Chem 29:552–558
Moqbel R, Durham SR, Shaw RJ, Walsh GM, Macdonald AN, Mackay JA, Carroll MP, Kay AB (1986) Enhancement of leukocyte cytotoxicity after exercise-induced asthma. Am Rev Respir Dis 133:609–613
Nagy L, Lee TH, Kay AB (1982) Neutrophil chemotactic activity in antigen-induced late asthmatic reactions. N Engl J Med 306:497–501
Papageorgiu N, Carroll M, Durham SR, Lee TH, Walsh GM, Kay AB (1983) Complement receptor enhancement as evidence of neutrophil activation after exercise-induced asthma. Lancet 2:1220–1223
Sherwood Burge P (1984) Aerosol challenge in lung disease. In: Clarke SW, Pavia D (eds) Aerosols and the lung: clinical and experimental aspects. Butterworths, London, pp. 225–250
Tomioka H, Ra C, Iwamoto I, Sato T, Kodama K, Yoshida S, Tanaka M, Yoshida S (1985) Arachidonic acid metabolism of human granulocytes from asthmatic patients. In: Hayaishi O, Yamamoto S (eds) Advances in prostaglandin tromboxane, and leukotriene research, vol. 15. Raven Press, New York, pp. 339–343
Zedda S, Cirla A, Aresini G, Sala C (1976) Occupational type test for the etiological diagnosis of asthma due to toluene diisocyanate. Respiration 33:14–21
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Valentino, M., Governa, M. & Fiorini, R. Increased neutrophil leukocyte chemotaxis induced by release of a serum factor in toluene-diisocyanate (TDI) asthma. Lung 166, 317–325 (1988). https://doi.org/10.1007/BF02714064
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DOI: https://doi.org/10.1007/BF02714064