Abstract
Extracellular nucleotides mediate a number of physiological responses through either ligand gated P2X or G protein-coupled P2Y receptors. To date, six P2Y receptor subtypes, P2Y1-P2Y6, have been cloned. We mapped the human P2Y6 receptor gene to chromosome 11q13.3-13.5. Oligonucleotide primers complementary to a part of the human P2Y6 receptor cDNA were used to amplify a region from genomic DNA from a panel of mouse/human somatic cell hybrid cell lines, each containing a single human chromosome. A PCR product of the expected size (714 bp) resulted from a single hybrid cell line containing human chromosome 11. The gene was further localized to a region of chromosome 11 using a subchromosomal hybrid panel containing different segments of chromosome 11. Based on the specific PCR product obtained and its Southern hybridization to the P2Y6 receptor cDNA, the human P2Y6 receptor gene was localized to chromosome 11q13.3-13.5. Previously, we have localized the P2Y2 receptor gene to human chromosome 11q13.5-14.1. This is the first report of the clustering of the P2 receptor genes. The clustering of these two P2Y receptor subtypes suggests a relatively recent expansion of the gene family by gene duplication.
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Literature Cited
Burnstock, G. (1978). A basis for distinguishing two types of purinergic receptors. InCell Membrane Receptors for drugs and hormones: A Multidisciplinary approach. (eds.) by R.W. Straub and L. Bolis. Raven Press, New York.pp. 107–118.
Fredholm, B.B., Abbracchio, M.P., Burnstock, G., Daly, J.W., Harden, T.K., Jacobson, K.A., Leff, P., and Williams, M. (1994).Pharmacological Reviews.46:143–156.
Webb, T.E., Simon, J., Krishek, B.J., Bateson, A.N., Smart, T.G., King, B.F., Burnstock, G., and Barnard, E.A. (1993).FEBS Lett. 324:219–225.
Lustig, K.D., Shiau, A.K., Brake, A.J., and Julius, D. (1993).Proc. Natl. Acad. Sci. U.S.A. 90:5113–5117.
Parr, C.E., Sullivan, D.M., Paradiso, A.M., Lazarowski, E.R., Burch, L.H., Olsen, J.C., Erb, L., Weisman, G.A., Boucher, R.C., and Turner, J.T. (1994).Proc. Natl. Acad. Sci. U.S.A. 91:3275–3279.
Akbar, G.K.M., Dasari, V.R., Sheth, S., Ashby, B., Mills, D.C.B., and Kunapuli, S.P. (1996).J. Receptor Signal Trans. Res. 16:209–224.
Webb, T.E., Henderson, D., King, B.F., Wang, S., Simon, J., Bateson, A.A., Burnstock, G., and Barnard, E.A. (1996).Mol. Pharmacol. 50:258–265.
Nguyen, T., Erbs, L., Weisman, G.A., Marchese, A., Heng, H.H.Q., Garrad, R.C., George, S.R., Turner, J.T., and O’Dowd, B.F. (1995).J. Biol. Chem. 270:30845–30848.
Communi, D., Pirotton, S., Parmentier, M., and Boeynaems, J.-M. (1995).J. Biol. Chem. 270:30849–30852.
Webb, T.E., Kaplan, M.H., and Barnard, E.A. (1996).Biochem. Biophys. Res. Commun. 219:105–110.
Chang, K., Hanaoka, K., Kumada, M., and Takuwa, Y. (1995).J. Biol. Chem. 270:26152–26158.
Communi, D., Parmentier, M., and Boeynaems, J.M. (1996).Biochem. Biophys. Res. Commun. 222:303–308.
Nicholas, Robert A., Watt, William C., Lazarowski, Eduardo R., Li, Qing, and Harden, Kendall T. (1996).Mol. Pharmacol. 50:224–229.
Dasari, V.R., Sandhu, A., Mills, D.C.B., Athwal, R.S., and Kunapuli, S.P. (1996).Som. Cell Mol. Genet. 22:75–79.
Ayyanathan, K., Webbs, T.E., Sandhu, A.K., Athwal, R., Barnard, E.A., and Kunapuli, S.P. (1996).Biochem. Biophys. Res. Commun. 218:783–788.
Glaser, T., Housman, D., Lewis, W.H., Gerhard, D., and Jones, C. (1989).Somatic Cell and Mol. Genet. 15:477–501.
Tokino, T., Takahashi, E-i, Mori, M., Tanigami, A., Glaser, T., Park, J.W., Jones, C., Hori, T-a, and Nakamura, Y. (1991).Am. J. Hum. Genet. 48:258–268.
Gerhard, D.S., Lawrence, E., Wu, J., Chua, H., Ma, N., Bland, S., and Jones, C. (1992).Genomics. 13:1133–1142.
Hunt, D., Van Heyningen, V., Jones, C., McConville, C., and Benham, F.J. (1994).Ann. Hum. Genet. 58:81–85.
Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989).Molecular Cloning. A laboratory manual, 2nd. Edition, Cold Spring Harbor Laboratory Press.
Sanger, F., Nicklen, S., and Coulson, A.R. (1977).Proc. Natl. Acad. Sci. U.S.A. 74:5463–5468.
Ayyanathan, K., Naylor, S.L., Kunapuli, S.P. (1996).Somatic Cell Mol. Genet. 22:419–424.
Samson, M., Soularue, P., Vassart, G., Parmentier, M. (1996).Genomics. 36:522–526.
Duncan, A.M.V., Anderson, L.L., Funk, C.D., Abramovitz, M., and Adam, M. (1995).Genomics. 25:740–742.
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Pidlaoan, L.V., Jin, J., Sandhu, A.K. et al. Colocalization of P2Y2 and P2Y6 receptor genes at human chromosome 11q13.3-14.1. Somat Cell Mol Genet 23, 291–296 (1997). https://doi.org/10.1007/BF02674420
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DOI: https://doi.org/10.1007/BF02674420