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Insect-transmitted vertebrate viruses: Alphatogaviruses

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Summary

Alphatogaviruses, of which Sindbis virus (SV) is the prototype, replicate to high titer in the laboratory both in mosquito cells and in vertebrate cells. By studying the replication of SV in mosquito cells as well as in vertebrate cells, we were able to obtain several viral mutants which have novel phenotypes and have contributed to our basic knowledge of this virus family. These include three host range mutants: SVAP15/21 which replicates normally in mosquito cells but is restricted in vertebrate cells and SVCL35 and SVCL58, which are restricted in mosquito cells but replicate normally in vertebrate cells. As well, two other mutants are described here: SVLM21, which can replicate in methionine-starved mosquito cells and SVMPA, which can replicate in mosquito cells treated with mycophenolic acid or ribavirin.

The causal mutations of both SVLM21 and SVMPA are within the sequence encoding the nonstructural protein nsP1; these and other findings have enabled us to associate the capping and methylation of the viral mRNAs with the nsP1 protein. Our work serves to emphasize that it is both worthwhile and important to study the replication of arthropod-borne viruses in cells derived from the arthropod host as well as in cells derived from the vertebrate host.

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References

  1. Brown, D. T.; Condreay, L. D. Replication of alphaviruses in mosquito cells. In: Schlesinger, S.; Schlesinger, M. J., eds. The Togaviridae and Flaviviridae. New York: Plenum Press; 1986:171–207.

    Google Scholar 

  2. Chamberlain, R. W. Epidemiology of arthropod-borne togaviruses: the role of arthropods as hosts and vectors and of vertebrate hosts in natural transmission cycles. In: Schlesinger, R. W., ed. The Togaviruses. New York: Academic Press; 1980: chapter 6.

    Google Scholar 

  3. Durbin, R. K.; Stollar, V. A mutant of Sindbis virus with a host-dependent defect in maturation associated with hyperglycosylation of E2. Virology 135:331–344; 1984.

    Article  PubMed  CAS  Google Scholar 

  4. Durbin, R. K.; Stollar, V. Sindbis virus mutants able to replicate in methionine-deprivedAedes albopictus cells. Virology 144:529–533; 1985.

    Article  PubMed  CAS  Google Scholar 

  5. Durbin, R. K.; Stollar, V. Sequence analysis of the E2 gene of a hyperglycosylated, host-restricted mutant of Sindbis virus and estimation of mutation rate from frequency of revertants. Virology 154:135–143; 1986.

    Article  PubMed  CAS  Google Scholar 

  6. Engelberg-Kulka, H.; Schoulaker-Schwarz, R. Stop is not the end; physiological implications of translational readthrough. J. Theor. Biol. 131:477–485; 1988.

    Article  PubMed  CAS  Google Scholar 

  7. Fallon, A. M.; Stollar, V. The biochemistry and genetics of mosquito cells in culture. Adv. Cell Cult. 5:97–137; 1987.

    CAS  Google Scholar 

  8. Harrison, S. C. Alphavirus structure. In: Schlesinger, S.; Schlesinger, M. J., eds. The Togaviridae and Flaviviridae. New York: Plenum Press; 1986:21–34.

    Google Scholar 

  9. Hsieh, P.; Robbins, P. W. Regulation of asparagine-linked oligosaccharide processing. Oligosaccharide processingAedes albopictus in mosquito cells. J. Biol. Chem. 259:2375–2382; 1984.

    PubMed  CAS  Google Scholar 

  10. Hubbard, S. C.; Ivatt, R. J. Synthesis and processing of asparaginelinked oligosaccharides. Ann. Rev. Biochem. 50:555–583; 1981.

    Article  PubMed  CAS  Google Scholar 

  11. Kowal, K. J.; Stollar, V. Temperature-sensitive host-dependent mutants of Sindbis virus. Virology 114:140–148; 1981.

    Article  PubMed  CAS  Google Scholar 

  12. Lemm, J. A.; Durbin, R. K.; Stollar, V., et al. Mutations which alter the level or structure of nsP4 can affect the efficiency of Sindbis virus replication in a host-dependent manner. J. Virol. 64:3001–3011; 1990.

    PubMed  CAS  Google Scholar 

  13. Malinoski, F.; Stollar, V. Inhibitors of IMP dehydrogenase prevent Sindbis virus replication and reduce GTP levels inA. albopictus cells. Virology 110:281–291; 1981.

    Article  PubMed  CAS  Google Scholar 

  14. Malinoski, F.; Stollar, V. Inhibition of Sindbis virus replication by ribavirin: influence of cultural conditions and of the host cell phenotype. Antiviral Res. 1:287–299; 1981.

    Article  CAS  Google Scholar 

  15. Mi, S.; Durbin, R.; Huang, H. V., et al. Association of the Sindbis virus RNA methyltransferase activity with the nonstructural protein nsP1. Virology 170:385–391; 1989.

    Article  PubMed  CAS  Google Scholar 

  16. Rice, C. M.; Levis, R.; Strauss, J. H., et al. Production of infectious RNA transcripts from Sindbis virus cDNA clones: mapping of lethal mutations, rescue of a temperature-sensitive marker, andin vitro mutagenesis to generate defined mutants. J. Virol. 61:3809–3819; 1987.

    PubMed  CAS  Google Scholar 

  17. Scheidel, L. M.; Durbin, R. K.; Stollar, V. Sindbis virus mutants resistant to mycophenolic acid and ribavirin. Virology 158:1–7; 1987.

    Article  PubMed  CAS  Google Scholar 

  18. Scheidel, L. M.; Durbin, R. K.; Stollar, V., SVLM21, a Sindbis virus mutant resistant to methionine deprivation, encodes an altered methyltransferase. Virology 173:408–414; 1989.

    Article  PubMed  CAS  Google Scholar 

  19. Scheidel, L. M.; Stollar, V. Mutations that confer resistance to mycophenolic acid and ribavirin on Sindbis virus map to the nonstructural protein nsP1. Virology 181:490–499; 1991.

    Article  PubMed  CAS  Google Scholar 

  20. Shope, R. E. Medical significance of togaviruses: on overview of diseases caused by togaviruses in man and in domestic and wild vertebrate animals. In: Schlesinger, R. W., ed. The Togaviruses. New York: Academic Press; 1980: chapter 3.

    Google Scholar 

  21. Singh, K. R. P. Cell cultures derived from larvae ofAedes albopictus (SKuse) andAedes aegypti (L). Curr. Sci. 36:506–508; 1967.

    Google Scholar 

  22. Stollar, V. Inhibition of Sindbis virus replication inAedes albopictus cells deprived of methionine. Virology 91:504–507; 1978.

    Article  PubMed  CAS  Google Scholar 

  23. Stollar, V. Togaviruses in cultured arthropod cells. In: Schlesinger, R. W., ed. The Togaviruses. Biology, structure, replication. New York: Academic Press; 1980:583–621.

    Google Scholar 

  24. Stollar, V. Approaches to the study of vector specificity for arboviruses-model systems using cultured mosquito cells. Adv. Virus Res. 33:327–365; 1987.

    Article  PubMed  CAS  Google Scholar 

  25. Stollar, V.; Malinoski, F. The effects of adenosine and guanosine on the replication of Sindbis and vesicular stomatitis viruses inAedes albopictus cells. Virology 115:57–66; 1981.

    Article  PubMed  CAS  Google Scholar 

  26. Strauss, E. G.; Strauss, J. H. Structure and replication of the alphavirus genome. In: Schlesinger, S.; Schlesinger, M. J., eds. The Togaviridae and Flaviviridae. New York: Plenum Press; 1986:35–90.

    Google Scholar 

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  1. Department of Molecular Genetics and Microbiology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, 675 Hoes Lane, 08854, Piscataway, New Jersey

    Victor Stollar

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Stollar, V. Insect-transmitted vertebrate viruses: Alphatogaviruses. In Vitro Cell Dev Biol - Animal 29, 289–295 (1993). https://doi.org/10.1007/BF02633957

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