Summary
Serum factors determine the extracellular requirement for both Ca2+ and Mg2+ for multiplication of normal human lung fibroblasts in vitro. Serum factors also affect the extracellular Ca2+ requirement for transformed fibroblasts but to a different extent than for normal cells. Transformed cells exhibit a reduced requirement for both Ca2+ and Mg2+ for multiplication. The apparent reduction in Ca2+ requirement of transformed cells is dependent on the level of serum factors in the medium. The reduced Mg2+ requirement for transformed cells is more striking than the loss of Ca2+ and independent of the level of serum factors in the medium. A sequential effector relationship among serum factors, Ca2+ and Mg2+, in a proliferative control system for normal cells is proposed. Alteration or bypass of an intracellular Mg2+-requiring process is proposed as a major lesion in the transformed cells. This alteration causes an observed loss of requirements for both Ca2+ and serum factors for the multiplication of transformed cells.
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This work was supported by Grant CA-15305 from the National Cancer Institute, Contract 223-74-1156 from the Bureau of Biologics, Food and Drug Administration, HEW Biomedical Research Support Grant S07RR05800, and the W. Alton Jones Foundation.
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McKeehan, W.L., McKeehan, K.A. Calcium, magnesium, and serum factors in multiplication of normal and transformed human lung fibroblasts. In Vitro 16, 475–485 (1980). https://doi.org/10.1007/BF02626460
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DOI: https://doi.org/10.1007/BF02626460