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Establishment of a macrophagelike cell line derived from U-937, human histiocytic lymphoma, grown serum-free

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Summary

A human macrophagelike cell line which grows in serum-free medium was established from a histiocytic lymphoma cell line, U-937. U-937 cells failed to differentiate into macrophagelike cells in serum-free medium plus 12-O-tetradecanoyl phorbol 13-acetate (TPA). Fibronectin and albumin in serum were necessary for differentiation of U-937 ceds into macrophagelike cells in enriched RDF medium supplemented with insulin, transferrin, ethanolamine, selenite, egg yolk lipoprotein (eRDF-ITESL medium). The established cell line exhibited several characteristic properties of macrophage such as nitroblue tetrazolium reduction, phagocytic and α-naphthylbutyrate-esterase activities, and tumor necrosis factor and interleukin 1 production. At present the cells have been continuously maintained in eRDF-ITESL medium through over 150 passages.

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Authors and Affiliations

  1. Institute of Food Chemistry, Department of Food Science and Technology, Faculty of Agriculture, Kyushu University, Hakozaki, Higashi-ku, 812, Kukuoka, Japan

    Zwe-Ling Kong

  2. Food Additive Laboratory, Food Protection and Preservation Division, National Food Research Institute, The Ministry of Agriculture, Forestry and Fisheries, 2-1-2 Kannondai, Tsukuba, 305, Ibaraki, Japan

    Misao Miwa & Kazuki Shinohara

  3. Laboratory of Cellular Regulation Technology, Graduate School of Genetic Resources Technology, Kyushu University, Hakozaki, Higashi-ku, 812, Fukuoka, Japan

    Hiroki Murakami

Authors
  1. Zwe-Ling Kong
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  2. Misao Miwa
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  3. Hiroki Murakami
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  4. Kazuki Shinohara
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Kong, ZL., Miwa, M., Murakami, H. et al. Establishment of a macrophagelike cell line derived from U-937, human histiocytic lymphoma, grown serum-free. In Vitro Cell Dev Biol 26, 949–954 (1990). https://doi.org/10.1007/BF02624468

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  • DOI: https://doi.org/10.1007/BF02624468

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