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Genetic analysis of plant regeneration in rice (Oryza sativa L.)

  • Plant Cellular and Developmental Biology
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Summary

A diallel study involving reciprocal crosses of four genotypes (IR8, 36, 54, and 64) was carried out to understand the genetic mechanism of plant regeneration from immature embryo-calli in rice. Regeneration frequency (% of calli that produced plants) varied from a high of 86% for IR54 to a low of 0% for IR36, while regenerated plants per embryo numbered from 0 to 7 when these same IR lines and the F1 hybrids were compared. Combining ability analysis revealed that both nuclear (with both additive and dominant effects) and cytoplasmic genes are important in controlling regeneration in rice. Parental lines and F1 hybrids with high ability to regenerate were identified.

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References

  1. Abe, T.; Futsuhara, Y. Varietal difference of plant regeneration from root callus tissues in rice. Jpn. J. Breed. 34:147–155; 1984.

    Google Scholar 

  2. Abe, T.; Sasahara, T. Variation in callus formation from seed in japonica, indica, and large grain varieties in rice. Jpn. J. Breed. 32:53–60; 1982.

    Google Scholar 

  3. Abe, T.; Futsuhara, Y. Genotypic variability for callus formation and plant regeneration in rice (Oryza sativa L.). Theor. Appl. Gen. 72:3–10; 1986.

    Article  Google Scholar 

  4. Bingham, E. T.; Hurley, L. V.; Kaatz, D. M.; Saunders, J. W. Breeding alfalfa which regenerates from callus tissue in culture. Crop Sci. 15:719–721; 1975.

    Article  Google Scholar 

  5. Brar, D. S.; Ling, D. H.; Yoshida, S. Plant regeneration from somatic cell cultures of some IR varieties of rice. In: “Biotechnology in International Agricultural Research.” International Rice Research Institute. 1985.

  6. Chu, C. C.; Wang, C. C.; Sun, C. S.; Yin, K. C.; Chu, C. Y.; Bi, F. Y. Establishment of an efficient medium for anther culture of rice through comparative experiments on nitrogen sources. Sci. Sin. 18:659–668; 1975.

    Google Scholar 

  7. Green, C. E.; Philipps, R. L. Plant regeneration from tissue culture of maize. Crop. Sci. 15:417–421; 1975.

    Article  Google Scholar 

  8. Griffing, B. Concept of general and specific combining ability in relation to diallel crossing systems. Aust. J. Biol. Sci. 9:463–493; 1956.

    Google Scholar 

  9. Hodges, T. K.; Kamo, K. K.; Imbrie, C. W.; Becwar, M. R. Genotype specificity of somatic embryogenesis and regeneration in maize. Biotechnology 4:218–223; 1986.

    Google Scholar 

  10. Hodges, T. K.; Kamo, K. K.; Becwar, M. R.; Schroll, S. Regeneration of maize. In: Zaitlin, M.; Day, P.; Hollaender, A., eds. Biotechnology in Plant Science: Relevance to Agriculture in the Nineteen Eighties. Orlando, Florida: Academic Press; 1985:15–33.

    Google Scholar 

  11. Jennings, P. R.; Coffman, W. R.; Kauffman, H. E. Rice Improvement. IRRI 1979:168.

  12. Kamo, K. K.; Becwar, M. R.; Hodges, T. K. Regeneration ofZea mays L. from embryogenic callus. Bot. Gaz. 146:327–334; 1985.

    Article  Google Scholar 

  13. Kempthrone, O. An introduction to genetic statistics. New York: Wiley and Sons; 1957.

    Google Scholar 

  14. Lai, K. L.; Liu, L. F. Further studies on the variability of plant regeneration from young embryo callus of rice plants (Oryza sativa L.). Jpn. J. Crop Sci. 55:41–46; 1986.

    Google Scholar 

  15. Lazar, M. D.; Baenziger, P. S.; Schaeffer, G. W. Combining abilites and heritability of callus formation and plantlet regneration in wheat (Triticum aestivum L.) anther cultures. Theor. Appl. Genet. 68:131–134; 1984.

    Article  Google Scholar 

  16. Suenaga, K.; Abrigo, E. M.; Yoshida, S. Seed derived callus culture for selecting salt-tolerant rice. Part I. Callus induction, plant regeneration, and variation in visible plant traits. IRRI Res. Pap. Ser. 79, II. pp. 1982.

  17. Tomes, D. T. Cell culture, somatic embryogenesis and plant regeneration in maize, rice, sorghum and millets. In: Bright, S. W. J.; Jones, M. G. K., eds. Cereal tissue and cell culture. Hingham, MA: Martinus/Nijnoff/W. Junk; 1985:175–203.

    Google Scholar 

  18. Yamada, Y.; Wang, Z. Q.; Tang, D. T. Plant regeneration from protoplast-derived callus of rice (Oryza sativa L.). Plant Cell Rep. 5:85–88; 1986.

    Article  Google Scholar 

  19. Ye, H. C. Studies on cell suspension culture and plant regeneration in rice. Acta. Bot. Sinica 26:52–59; 1984. In Chinese with English summary.

    Google Scholar 

  20. Willman, M.; Schroll, S.; Hodges, T. K. Inheritance of somatic embryogenesis and plant regeneration from primary callus in maize. In vitro: Plant Cellular and Development Biology: In press.

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Authors and Affiliations

  1. Department of Botany and Plant Pathology, Purdue University, 47907, West Lafayette, Indiana

    Jianying Peng & Thomas K. Hodges

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  1. Jianying Peng
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  2. Thomas K. Hodges
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Peng, J., Hodges, T.K. Genetic analysis of plant regeneration in rice (Oryza sativa L.). In Vitro Cell Dev Biol 25, 91–94 (1989). https://doi.org/10.1007/BF02624416

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  • DOI: https://doi.org/10.1007/BF02624416

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