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Culture conditions for arresting and stimulating the proliferation of a rainbow trout fibroblast cell line, RTG-2

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Summary

Conditions for arresting and stimulating the proliferation of the rainbow trout fibroblast cell line RTG-2 have been examined and the time course of events after stimulation determined. Quiescent populations were achieved in two ways. Cultures grown to confluency without a medium change for at least 7 d had fewer than 5% of the cells in S phase and few mitotic figures. Cultures deprived of serum, which could be done for up to 3 d without a loss in cell number, also achieved quiescence. After 3 d without serum, less than 1% of cells were in S phase and mitotic figures were infrequent. Addition to these cultures of fresh serum-containing medium brought about the synchronous entry of cells into S phase and mitosis. For cultures in which either the medium had been changed after 7 d without a change or serum-containing medium had been added after 3 d of serum deprivation, DNA synthesis increased after a lag period of 20 to 24 h, was pronounced between 30 and 45 h, and then declined. This was followed by a peak in the mitotic index. These protocols for arresting and subsequently stimulating RTG-2 proliferation should allow the G1-S transition to be studied in a representative of teleosts.

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Authors and Affiliations

  1. Department of Biology, University of Waterloo, N2L 3G1, Waterloo, Ontario, Canada

    L. E. J. Lee, A. Martinez & N. C. Bols

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  1. L. E. J. Lee
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  2. A. Martinez
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  3. N. C. Bols
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This research was supported by Natural Sciences and Engineering Research Council of Canada grant to N. C. B.

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Lee, L.E.J., Martinez, A. & Bols, N.C. Culture conditions for arresting and stimulating the proliferation of a rainbow trout fibroblast cell line, RTG-2. In Vitro Cell Dev Biol 24, 795–802 (1988). https://doi.org/10.1007/BF02623650

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