Summary
The rat hepatoma cell line, H4-II-E, was grown serially over a I-year period and about 30 passages in arginine-, glutamine-, and tyrosine-deprived and ornithine-supplemented Eagle's mininum essential medium with no supplements other than biotin. The adapted cel line, R-Y121B, proliferates in the above mentioned medium with a doubling time of about 4 days and maintains hepatic “marker” enzymes such as tyrosine aminotransferase, phenylalanine hydroxylase, and all the enzymes of the urea cycle.
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This work was supported in part by Grant-in-Aid for Cancer Research 301050 and Science Research Grant 337013 from the Ministry of Education, Science and Culture, Japan.
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Niwa, A., Yamamoto, K., Sorimachi, K. et al. Continuous culture of reuber hepatoma cells in serum-free, arginine-, glutamine-and tyrosine-deprived chemically defined medium. In Vitro 16, 987–993 (1980). https://doi.org/10.1007/BF02619337
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DOI: https://doi.org/10.1007/BF02619337