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Development in vitro of epithelial-cell monolayers derived from fetal rat pancreas

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Summary

Purified epithelial-cell monolayers were generated in vitro from explants of fetal rat pancreas. The extent of the development of the epithelial monolayer, as determined by planimetric analysis, was enhanced by the application of two methodological procedures: (a) preincubation of fetal pancreas in situ at 27° C for 5 hr prior to dissection and explantation; and (b) incubation of the explants in medium containing a high concentration (50% to 70%) of fetal bovine serum. By utilizing such culture conditions, sheets of contiguous epithelial cells, with little or no peripheral fibroblastic contamination, were maintained for 9 days. Whereas the majority of cells within the monolayer had morphological characteristics of pancreatic ductal cells, endocrine cells were identified by the specific immunocytochemical localization of insulin and glucagon. In addition, insulin could be detected in the incubation medium throughout the course of the experiment. The simplicity of this preparation offers some advantages over other techniques including reduced chance of contamination and reduced cellular damage or death. It provides a model for future studies directed toward developing individual cell strains derived from pancreatic epithelial cells.

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Authors and Affiliations

  1. Department of Anatomy, University of Minnesota, 55455, Minneapolis, Minnesota

    Daniel H. Wallace & Orion D. Hegre

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  1. Daniel H. Wallace
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  2. Orion D. Hegre
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These studies were supported in part by NIH Grants HD 00412 and GM 114, and a grant from the American Diabetes Association—Minnesota Affiliate.

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Wallace, D.H., Hegre, O.D. Development in vitro of epithelial-cell monolayers derived from fetal rat pancreas. In Vitro 15, 270–277 (1979). https://doi.org/10.1007/BF02618951

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